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...December 2015 we held our first intro to bacterial transformation course (read our blog post on it and to see...consisting of over twenty members and volunteers. Transforming citizens into scientists Anyone can become a...dedicated time at BosLab, he became our bacterial transformation guru and instructor for our intro to biohacking...BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig...Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments for discovering novel...Addgene and are eager to do our first yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami...

...force can be generated by conformational changes in proteins? How can information propagate through a structured...monomers respond to pH changes by undergoing local conformational changes. These tiny movements are then amplified...these applications, purified R bodies could also perform as actuators in microelectromechanical systems ...

... the then RNAi Platform, which has since evolved into the Genetic Perturbation Platform and encompasses...Associate Director of the Genetic Perturbation Platform at the Broad Institute and has worked with many...The model that the Broad was building that had Platforms with a capital ‘P’ focused on technology ... has...enjoys working on technology development under the Platform model at the Broad Institute because they are ...CRISPR Learn more about the Broad Institute and the Platform Model. ...

...engineers regularly use the platform, why scientists are using the platform, and tips for getting the most...from Nature Jobs Blog While Twitter is a popular platform for scientists, it’s not the only tool in a scientist... Facebook. For each of the three social media platforms, there’s some statistics on what percent of scientists...research potential future employers and to arrange informational interviews. 4. “Tips for Scientists Using Social...given you tips for using various social media platforms, and 3) helped you fit social media into your ...

...open repository of plasmids and other necessary information that’s required to do research. Paratore: What...scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...: The promise there is the ability share the information, making it available to everyone, that creates...breakthrough and innovation. With any kind of open platform, there is the potential for increased speed and...certified public accountant (CPA), certified information systems auditor (CISA), and certified health ...both the Internal Institute of Auditors and the Information Systems Audit and Control Association. Lastly...

...use. Or you can really capitalize on the dye’s performance and use super-resolution or single-molecule techniques...fluorescent ligand and see the results right away. Or perform pulse-chase experiments by sequentially adding ...-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta) and capping protein (549-CP...priority, SNAP-Tag or HaloTag have been shown to perform differently for different ligands (Wilhelm et al...super-resolution imaging, HaloTag gives better performance than SNAP-tag (Erdmann et al., 2019). Check out...new ligands for these systems, enhancing their performance and ease of use. With so many possibilities from...regulation of actin barbed end assembly by twinfilin, formin and capping protein. Nature Communications, 14(...

...ways scientists communicate with each other: from formal mechanisms like publishing a research article, ...or submitting patent or grant applications; to informal settings like lab meetings, social media, coffee...blogging and more. These each have different goals, formats, and processes, and they provide different amounts...one of the key routes for researchers to share information about the emerging COVID-19 pandemic quickly ...before, submitting the manuscript to a journal for formal peer review. Some organizations, including some...preprints are eventually published in journals after formal peer review, with the earlier attention and feedback...

...through the literature until you have all of the information you need. Reading primary research literature...written. By nature, scientific literature is information dense and since it continually builds on previous...The introduction should give you just enough information to allow you to understand the research question..., statistics, clarity in images etc)? Did they perform all of the appropriate controls? Refer back to ...Are there any red flags about how the work was performed that could influence the results? The peer-review...misinterpretations. After all, the science was performed, written and evaluated by humans. Keeping up with...

...intracellular parasites and natural vehicles for genetic information. Therefore they make excellent tools for genetic...same gene products for packaging, however, the isoforms of these proteins, as well as the viral LTRs differ...be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with ...Consortium, also known as the Genetic Perturbation Platform, to produce their shRNA library to support functional... with an shRNA sequence of your choice. More information and a detailed protocol on how to use the pLKO...www.genome-engineering.org for the most up-to-date information. Check our CRISPR guide and blog to learn more...

... to be recruited per targeted gene. Performance SunTag performs better than first generation activators...elongation, depending on which sequence is targeted. Performance dCas9-VP64 activation is generally thought of...additional activation domains (HS1 and p65). Performance When targeting single genes, SAM consistently...in tandem to recruit transcription factors. Performance Generally, VPR is found to have significantly... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

...the glut of information, but it’s not in the best interest of any company to hide information about their... surprised to discover that the most crucial information about a product is printed right on the tube ...pages on website are another great source of information. At least at Addgene, the questions in our FAQ...the resources to recapitulate the experiments performed by our thousands of depositors. That means their...specifics. We can help you faster if we get as much information as possible about the issue. For Addgene support...

...BioRender.com. Transformation Grade DNA (low quality/low quantity) – Bacterial transformation protocols require...kits are quick, easy, and don’t involve phenol-chloroform.  The default option is to use the kit that ...highest-competency cells you will still struggle to get transformants if the plasmid you are using is completely degraded...quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep, midiprep...bead- and column-based DNA purification methods perform well in terms of quality and yield. Bead-based ...provide specs, protocols, and quality control information, but the level of detail can vary. If you’re ...

...found that many researchers feel that they lack formal training in a variety of transferable skills. At...stayed until he graduated. Years later I attended a formal training for new managers and I had a “Eureka” ... have a much better chance of creating a high-performing team - which is every manager’s dream. Learn ...often associate feedback with end-of-the-year performance review, but feedback should be given throughout...the year and in a timely manner. If someone’s performance or behavior is inadequate, you need to address... “bad” managers and I've learned from both. The former will empower you, keep you motivated and help you...

...cancer dependency database used to make this information accessible to the research community. Sean Misek... to guides in the Broad’s Genomic Perturbation Platform Avana library (used by the Cancer Dependency Map...of the dependencies detected. Given this new information, Misek notes that in theory, if the DepMap analysis...used CRISPR screens, they had also found the information they needed to begin correcting the issue. Working...and Adam Brown from Broad's Genetic Perturbation Platform to design a new library that is agnostic to ancestry... are working with Broad's Genetic Perturbation Platform to modify the CRISpick algorithm to prevent guides...

...challenge at hand and help resolve it through well-informed recommendations. Healthcare consulting firms that...to get a large amount of market research done to inform a go/no-go decision in a very short amount of time...pressure to maintain consistently high levels of performance, and occasionally being faced with “shifting ...crucial to how our clients’ products will end up performing in the market. Hence, as a PhD, an MD, or someone... successful therapeutics can be a great fit for former healthcare consultants. I have even seen consultants...especially helpful since they both provide up-to-date information about the industry, recruiting processes, and...

...set of primers and sgRNA that experimentally outperformed the sequences generated separately by other ...Drew Endy said in the 60 Minutes segment that our platform isn't limited to Lyme and could detect essentially...of techniques like PCR, gel electrophoresis, transformation, and how CRISPR works before we even apply ...designs, but making sure those designs actually perform in the wet lab is significantly harder. We had ... framework actually translated to real-world performance. Can you tell us a highlight from your competition... on pollinator health, specifically working on Deformed Wing Virus in honeybees. DWV is one of the most...

...Schematic of BeadScan platform for screening candidate biosensor variants for performance under two-photon ...high-throughput screen to select variants based on performance metrics specifically for two-photon microscopy...Near-infrared (NIR) fluorescent proteins promise better performance for in vivo use and deep-tissue imaging. Vladislav...concentrations of lactate. The microfluidic screening platform developed here is also a promising approach for...produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable under ...

...CSV file, anytime. The CSV is a rich source of information and has data on the date, material, requesting...requesting PI & organization, and country. This information can be of personal interest to the depositor, but...interesting to parse through, aggregation of this information can provide valuable insight to the most requested...why I turned to dashboards. Dashboards provide information in a concise and clear way that is accessible... the public sharing of aggregated data only, information on requesting PI(s) & organization(s) is not ...

...asked the individuals behind some of these new platforms what their motivations were for developing these...COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…).  How to put the wider...Resources Canada is a database that provides information about reagents, funding and volunteer opportunities...this program is based in Canada, much of the information is applicable to researchers worldwide. “COVID...research in their field including datasets and platforms for discussing new developments, and more. As ...

...Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in a wide...recombination repair pathway. Reagents required to perform pha-1 co-conversion are available through Addgene...ensuring the PAMs remain on the chromosome after DSB formation, was most efficient (Farboud et al., 2019). Using... C. elegans. The C. elegans work on small RNAs informed and drove work in countless other systems, whereas...CRISPR technology into C. elegans has been a transformative innovation for our community. Developments ... genomic locus (CRISPR-ChAP-MS) could further transform the range of experiments imaginable in C. elegans...