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We narrowed to 833 results for: ins

Showing: 341 - 360 of 833 results

  1. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...target pathogenic proteins, as well as in the lab to deplete expression of proteins of interest. RNAi ...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...agriculture as species-specific pesticides and insecticides, an advancement over previous chemical versions... these structures can in turn lead to genome instability. Thus, it’s important to strike a balance between..., I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with RNA/DNA hybrids. Genome Res...10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat...

  2. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...funding from a variety of government and academic institutions. New graduate students may assume that their...given problem, Empowers the writer, And offers insight into a career as a Professor. Step 1: Your ... to read and fully understand the RFA. The RFA contains all of the important information required for ...the mission of the application and the funding institution. Moreover, avoidance of ‘to be’ verbs creates...time to review the literature and offer educated insight into what the desirable features of future technologies... with the description of the RFA and funding institution. Emphasizing how your solution fits into the ...funded proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended protocol...

  3. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...abilities, we’re gaining new ground in the fight against cancer in its many forms. The dichotomous interactions...major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition by CD8+...formation and metastasis, block the cell-surface proteins that help escape immuno-surveillance, and curb...Antibodies that block cell-surface tolerance-producing proteins are one of the most commonly used immunotherapeutic...sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...genome engineering have played a huge role. For instance, researchers are genetically altering tumor cells...cells and immune cells to knockout proteins involved in immune tolerance. Recently, China spearheaded ...

  4. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow at The Sainsbury Laboratory...blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early...audience’s applause with your performance. When your institute opens its doors to the public, for example at ...your own blog, you can probably write for your institute’s blog. The Wellcome Trust blog or the John Innes... careers are in advance of yours, but also get inspired and meet peers. Join societies like National Science...provides on-site courses in public engagement for institutions and the Royal Society gives training in media...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...ensuring that the plasmid they ordered contains the correct inserts, tags, promoters, and other critical...markers, and fusion proteins listed for each plasmid  If the plasmids pass all inspection points, they are...ensure our assembly result is accurate The gene/insert or any major feature highlighted by the deposited...called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided. If no...assembly in Geneious can still provide additional insights to resolve the assembly and determine if there...

  6. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...pursue leadership opportunities at the local, institutional, regional, and national levels. These skills... But how does one gain such experience? Your institution may offer occasional workshops on leadership ...Harvard Medical School and then Boston University (institutions where I’ve worked as a postdoc). I soon realized...that many of the issues faced by postdocs and institutions are not local but are national issues. I applied...requirements of the new Board Members. In some instances I felt like I was going back to previous formats...every year it organized a “Committee Leadership Institute” (CLI) where each Committee Chair and Vice Chairs...the Burroughs Wellcome Trust. These “leadership institute’ weekends would entail intense training to develop...

  7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...detector plants, we expected to see that same anger. Instead, social media comments were more often along the...that.” That was interesting. If people could be against GMOs as food but support GMOs that result in a ...business training. The money is a drop in the bucket against the funds needed to build a flower that changes...business enterprises, he can be found climbing mountains or playing with his two cats. Keira Havens grew...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...

  8. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...something done not by people wearing a lab coat, but instead by people wearing business suits discussing multimillion...that means you don’t. In this mindset, one of us wins, the other loses. Although this approach to negotiation...winner and a loser when negotiating, what if we instead approached it as a creative problem-solving exercise...point you toward a creative solution where everyone wins. What are the underlying reasons for this person...some data for an upcoming conference deadline. Instead of arguing over who gets the spot (a zero sum result...Coming up with creative solutions where everyone wins can initially feel more difficult because of the...can I offer them to make it a win-win situation instead of just extra work for them? As a business development...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...stem from the heart beating at an irregular or insufficient level. The heartbeat – a muscle contraction ...structurally into 2 main groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors...that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport, not ...membrane due to the addition of several targeting domains. The quencher and voltage detector in this system...viruses also have various serotypes – the surface proteins that confer affinity for a target – and this serotype...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them for targeting... Viral Vectors 101: Optogenetics Fluorescent Proteins 101: Introduction to FRET ...

  10. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... sequences, such as mismatches, deletions, or insertions. We use blastp or blastx to compare our sequencing...database “Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...Non-redundant protein sequences (nr)” database as it contains the largest number of protein sequences. Just ...customization. Similar to nucleotide sequences, proteins often have repeated or highly homologous regions... and free up more time for using your plasmids instead! All BLAST images are modified screen shots from...101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality Control Learn about...

  11. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... things like genomic DNA, RNA, proteins, salts, ethanol, or endotoxins. The most common method for checking...to be free of contaminants, such as bacterial endotoxins, that could affect your culture’s viability. ...plasmids – If you’re extracting plasmids from yeast, instead of bacteria, a few manufacturers (such as Zymo ...struggling with a purification, reading the full instruction manual will help explain the rationale behind... helpful to select a kit with easy-to-follow instructions and extensive references for troubleshooting... compare kits at a quick glance, Table 3 below contains details regarding kits from a variety of manufacturers...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

  12. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... contestants!           View this post on Instagram                     A post shared by Xi Chen...December 12, 2022          View this post on Instagram                     A post shared by 草方 (@gracie_fang...take photos, and then share them on Twitter or Instagram with #DeckTheLab and tagging us, @addgene to be...get you out of.           View this post on Instagram                     A post shared by Xi Chen...picture or video for either contest on Twitter or Instagram by December 31, 2022.  Follow @Addgene, use the...and reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light...

  13. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...reprogramming efficiency through the expression of a shRNA against p53, a modified reprogramming factor cocktail, ...are preferred. This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes...disruption of functional genes at the site of insertion and which can potentially promote tumorigenesis... been recorded in past gene therapy trials and remains a safety concern in the clinical field [4]. Three...Antigen-1 (oriP-EBNA-1) system where each vector contains a viral origin of DNA replication, OriP and the... pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1 37624 transient EBNA-1   ...Road Ahead." Journal of Alzheimer's disease & Parkinsonism 6.5 (2016). PubMed PMID: 27882265. PubMed Central...

  14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...Singletons in their bins.  The next component of Binning Singletons is when we bring all the bins together at ...invaluable to me as a bench scientist working at an institution where training opportunities weren’t always available...their own labs.  Fried chicken with a side of inspiration  I look back at the meeting with such fondness...for DNA that group similar sequences together in bins, we thought that binning Singletons together would... and time mentors spend with Singletons in their bins. We also sought expertise from many with experience...media. Joe is a lapsed marathon runner, father of twins (AND a singleton), and is proudly "too weird to ...

  15. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...diagnosed this year). Research efforts in mesothelioma instead rely upon private donations. Many people think ...However, there is no question that asbestos exposure remains the single-most important factor in developing ...do not work very well. The best option in such instances is palliative care, which helps patients manage...detection techniques and therapies emerge into mainstream use, they will have a much greater impact on ... good detection and treatment get, there still remains the problem of prevention. Asbestos exists pretty... and the president of the organization, Linda Reinstein – whose husband passed away from mesothelioma ...with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016 in the U.S. Senate...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels (which had therefore been ... light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential labeling by TurboID...2023 bioRxiv (B-C). They were able to label proteins moving to and from the nucleus, ER, mitochondria...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...frequency of events, instead of relying on clustered dots. In a contour plot, each line contains the same number...machine reads/interprets the signals, the axis is instead divided into positive and negative signal at a ... a ‘gate’; it tells the machine that everything inside the gate is one population and everything outside...which measure light that “scatters” past the cell, instead of fluorescent markers. There are various types...to fluorescent markers and/or cellular dyes and stains. These will be used in a series of one- or two-...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... apply for the position. This post on Business Insider nicely summarizes good job fair practice. Career...get online and take a look at the company or institution’s career page, where you’re likely to find current...conference or job fair, or it may be someone at your institution’s career center. Alternatively, it might even ...other graduate students and post-docs from your institution have gone, and try sending them a professional... professional development, not count on your institution, program, or adviser to do it for you. Expose...if one exists. Get online! Review company and institution career pages. Make yourself visible on LinkedIn... Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014...

  19. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...DNA left behind after another bacterium died for instance). These three mechanisms of acquired drug resistance...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be... time is needed for the drug to work… if the instructions for treatment aren’t followed closely, you could...in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our Microbiology Pages Additional...

  20. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...approach, which focuses on validating antibodies against markers of the human blood leukocyte populations...cases, we would recommend a knockdown approach instead. In this approach, you’re looking for an antibody...permanent knockdown, consider using shRNA vectors instead. With both siRNA and shRNA, it can be difficult...induce/suppress the expression of many related proteins, not just your protein of interest, which can ...interest, which can be difficult to find for many proteins. In practice, it was necessary for us to combine...primary blood leukocytes. The file shared also contains information about the labeling pattern of several...on human blood leukocytes, including antibodies against these markers. The 371 CD markers characterized...
Showing: 341 - 360 of 833 results