We narrowed to 363 results for: nsf
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TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....and decarboxylases. These enzymes catalyze the transfer of carboxyl groups to organic acids to produce...
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Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....Arabidopsis. There are also important tips for plant transformations and the use of Arabidopsis in the classroom... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....retinal gene therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out ... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....downstream applications, like an in vivo adoptive transfer of those CD8+ T cells, require the cells to be... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....fragmented into smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....genome. While this may effectively serve as a transfection or transduction control, a different gRNA would... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....equal loading across samples and equal protein transfer from the gel to the membrane. A loading control... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....gfaABC1D-Driven Transgene Expression in AAV-Mediated Gene Transfer. Molecular Therapy - Nucleic Acids, 8, 13–25. ... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality." Neuron... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m.... With the ability to produce neuron specific transfection using Cre-lox, this new method could allow for... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....the modified allele is selectable, it can be transferred to a different genetic background via P1 transduction... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m.... Non-Academic Career Decisions Check out our transferable skills guide Resources on Addgene.org Learn... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....capping enzyme superfamily of covalent nucleotidyltransferases." Current opinion in structural biology... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m.... a targeting sequence for the adenine phosphoribosyltransferase (APT) gene, which is conserved in many... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....a process called Fluorescence Resonance Energy Transfer (FRET). Figure 1: Emission spectra and... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....available, instructions for obtaining a material transfer agreement, and list of vendors (such as the vector...