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We narrowed to 352 results for: plasmids 101

Showing: 341 - 352 of 352 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...Activation. Cell 159:647–661 . https://doi.org/10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino...Screens. Cell Systems 6:555-568.e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015...Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Wong N, Liu W, Wang X (2015)...

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...Toxicological Methods, 64, 233–237. https://doi.org/10.1016/j.vascn.2011.06.001 More resources on the Addgene...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I. V., Teyssier...Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E....

  4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...the MG-2I-dL5** FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation...biology. Trends in Cell Biology,18(9), 443-450. doi:10.1016/j.tcb.2008.07.001. PubMed PMID: 18706812. PubMed...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...Microbiol Methods. 2006 Jun;65(3):476-87. doi: 10.1016/j.mimet.2005.09.007. Epub 2005 Oct 10. PMID: 16216354...

  6. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...neuroscience. 2011;34:389-412. doi: 10.1146/annurev-neuro-061010-113817. PMID: 21692661.  3. Bernstein JG, Boyden... cognitive sciences. 2011;15(12):592-600. doi: 10.1016/j.tics.2011.10.003.  PMID: 22055387;  PMCID: PMC3225502...Progress in brain research. 2012;196:193-213. doi: 10.1016/B978-0-444-59426-6.00010-0. PMID: 22341327; PMCID... consumption. Neuron. 2012;73(6):1184-94. doi: 10.1016/j.neuron.2012.02.016. PMID: 22445345; PMCID: PMC3314244...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...Trends Mol Med. 2018, S1471-4914(18)30123-0. DOI: 10.1016/j.molmed.2018.06.006. PubMed PMID: 29983335. 9....

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is... Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513. PubMed Central PMCID: PMC1368960. 10. Denning...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...Sequencing. Cell Stem Cell 15:27–30 . https://doi.org/10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne S, Yang...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...Chemistry & Biology 22:169–174 . https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA...
Showing: 341 - 352 of 352 results