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We narrowed to 359 results for: plasmids 101

Showing: 341 - 359 of 359 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    .... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...Precision Genome Editing. BioRxiv. https://doi.org/10.1101/354480 Ghanta KS, Mello CC (2020) Melting dsDNA...elegans. Methods 121–122:86–93 . https://doi.org/10.1016/j.ymeth.2017.03.023 Paix A, Wang Y, Smith HE, Lee...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable,...Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin, B. A., Adams, S...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...get right. Here at Addgene, we use a linearized plasmid standard. However, even when the standard is made... could end up with a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/mL and both results ... Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...on the Addgene blog Read blog posts related to plasmid protocols Find protocols for purifying DNA without...BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin JM, Przybyla AE, ...Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006...

  5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors... Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028. PubMed PMID: 20144757. PubMed... and Non-Mammalian Systems, Part A, 33-42. doi:10.1016/s0076-6879(08)03203-5. PubMed PMID: 19185711. Additional...

  7. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...23;161(3):674-690. doi:10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...Activation. Cell 159:647–661 . https://doi.org/10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino...Screens. Cell Systems 6:555-568.e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015...Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Wong N, Liu W, Wang X (2015)...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...Toxicological Methods, 64, 233–237. https://doi.org/10.1016/j.vascn.2011.06.001 More resources on the Addgene...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I. V., Teyssier...Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E....

  11. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...the MG-2I-dL5** FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation...biology. Trends in Cell Biology,18(9), 443-450. doi:10.1016/j.tcb.2008.07.001. PubMed PMID: 18706812. PubMed...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...Microbiol Methods. 2006 Jun;65(3):476-87. doi: 10.1016/j.mimet.2005.09.007. Epub 2005 Oct 10. PMID: 16216354...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...neuroscience. 2011;34:389-412. doi: 10.1146/annurev-neuro-061010-113817. PMID: 21692661.  3. Bernstein JG, Boyden... cognitive sciences. 2011;15(12):592-600. doi: 10.1016/j.tics.2011.10.003.  PMID: 22055387;  PMCID: PMC3225502...Progress in brain research. 2012;196:193-213. doi: 10.1016/B978-0-444-59426-6.00010-0. PMID: 22341327; PMCID... consumption. Neuron. 2012;73(6):1184-94. doi: 10.1016/j.neuron.2012.02.016. PMID: 22445345; PMCID: PMC3314244...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...Trends Mol Med. 2018, S1471-4914(18)30123-0. DOI: 10.1016/j.molmed.2018.06.006. PubMed PMID: 29983335. 9....

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is... Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513. PubMed Central PMCID: PMC1368960. 10. Denning...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...Sequencing. Cell Stem Cell 15:27–30 . https://doi.org/10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne S, Yang...

  19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...Chemistry & Biology 22:169–174 . https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA...
Showing: 341 - 359 of 359 results