We narrowed to 430 results for: sequence

1. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   Published

   Jan. 25, 2018, 2:09 p.m.

   ...which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin to a lysine residue...

2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   Published

   Nov. 20, 2018, 8:17 p.m.

   ...description, how the plasmid was generated, validated sequence information, and more. The plasmid page also helps...

3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   Published

   July 7, 2015, 3:36 p.m.

   ...and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome. Figure 1:...

4. Advice for Scientists Starting a Lab

   Type

   Blog Post

   Published

   April 29, 2014, 1:43 p.m.

   ...interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results to companies.” Take...

5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   Published

   April 7, 2022, 1:15 p.m.

   ...anchoring domains – GPI and SGZ. Fig: 4 Sequence schematics for PDGFR, GPI, and SGZ display constructs...

6. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ... data our depositors share and enrich it with sequencing data; as a result, the materials in our catalog...

7. With Our New Viral Service, We're Taking CRISPR Further!

   Type

   Blog Post

   Published

   Dec. 6, 2016, 3:30 p.m.

   ...must amplify the library, use next-generation sequencing to ensure that the library has retained gRNA ...

8. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching space ...

9. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...use to tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate...

10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ...  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends: Sequence based input is by far the...

11. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...recombination by finding acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas ...

12. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...expressing a different isotype, the intervening DNA sequence is deleted, which places the variable region next...

13. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...generate antibodies against, or a unique peptide sequence derived from this protein. At the same time, an...

14. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...terminator regions and are slightly different in sequence. Both LTRs do share common repeat regions in addition...

15. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ...editors capable of editing previously uneditable sequences. Four DNA base swaps, so called point mutations...

16. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...be programmed to bind tightly to arbitrary DNA sequences and have been shown to achieve ~100 fold repression...

17. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...caspase) cleavage site (DEVD). Upon cleavage of the sequence, β10 and β11 can rearrange to their normal antiparallel...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ... mVenus linked to a mitochondrial localization sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted...

19. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...collection reported to date, containing all 620 sequences of probable mature human miRNAs. Weiss’s collection...

20. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...into the genome randomly, while others use attP sequence docking sites so that you can control exactly ...