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Showing: 341 - 360 of 422 results

  5. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...terminator regions and are slightly different in sequence. Both LTRs do share common repeat regions in addition...

  9. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...recombination by finding acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas ...

  10. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...expressing a different isotype, the intervening DNA sequence is deleted, which places the variable region next...

  11. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...generate antibodies against, or a unique peptide sequence derived from this protein. At the same time, an...

  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... the dCas9-CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the...photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a double...

  14. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...product on an agarose gel, then cutting out and sequencing the band for verification. Primer dimers will...uMelt is an online tool that uses the  amplicon sequence and thermodynamic and experimental parameters ...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA) are removed, resulting...

  16. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ... affinity chromatography against an amino acid sequence, or tag, on the protein. While a variety of methods...

  18. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...transcriptional units. Individual parts are defined DNA sequences with specific functions. Most individual parts...
Showing: 341 - 360 of 422 results