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We narrowed to 718 results for: tat

Showing: 341 - 360 of 718 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... pathway of interest, but there can be a lot of static since other signaling networks can also be non-....” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure 1: Photoswitchable...turn off psRaf1). After this, the phosphorylation status of MEK1/2 was tracked at 5 minute intervals via...

  2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...complicating the interpretation. Because of this, these methods only give a qualitative view of dimerization...molecules based on size is ultracentrifugation. Sedimentation equilibrium analytical ultracentrifugation yields...

  3. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...for compliance. However, 10 days prior to the implementation date, our data indicated that, while 69% of...the lapsed mandate (or are being compelled at the state level, in the case of some public institutions) ...continuing to reach out to institutions with a statement indicating that we encourage them to keep raising...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...altering gut microbiome members in their native habitat (Ronda et al., 2019). They called their method ...the addition of standards, the assay is semi-quantitative and can approximate mRNA concentration within...infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed ...

  5. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...assay for a native (folded) protein, such as immunoprecipitation, the epitope may not be accessible for binding... down in a variety of ways. First, review the citations. An antibody that has been cited thousands of ...antibody to that of the anti-tag antibody; and immunoprecipitation-mass spectrometry strategies that isolate...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...vector with half the size of the ssAAV genome has a mutation in the terminal resolution site (TRS) to form ...genome with wild-type ITRs at the both ends and mutated ITR at the center of symmetry. After uncoating ..."Development and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 ...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...to start an open science nonprofit that would facilitate the sharing of plasmids between scientists. The... r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process, which... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction sites used...most sequence assembly errors occur), deletions, mutations, in-frame protein fusions, and multiple cloning...cases, we deal with multiple inserts or internal mutations that require more than two sequencing reactions...

  9. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...A professional coach will provide an initial consultation meeting or call for free.  Take advantage of...affordable for you. There is also a group The Dissertation Coach based in Illinois. A few of the coaches...in this group have scientific backgrounds. For a stated purpose of transitioning a scientist from academia...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...useful for neuronal tracing, it has two practical limitations. First, these high levels of viral replication...an enveloped virus. In general, viral envelopes dictate infection of target cells through their interactions...evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb. Proc Natl Acad Sci U ...

  11. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...conditions (individually versus in groups), health status, diet, cage enrichment, stress level, origin (vendor...of channel protein, they can trigger neuronal excitation or inhibition upon light (or drug) application...situations such as learning and memory or emotional states, for example anxiety or depression. In upcoming...

  12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...which is then implanted into a female animal for gestation. Nucleases that induce double-stranded DNA breaks...which is then implanted into a female mouse for gestation. All three can introduce multiple base pair deletions...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

  13. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...review analyzing and explaining the antibody crisis states that up to 50% of all commercially available antibodies... technology, it is important to show through qualitative data that they are a long-term solution to reproducibility... above) and literature suggest, providing an expectation and comparison on the antibody signal’s location...

  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... if you want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region... hand, if you are trying to knock-in a patient mutation or need to make a precise amino acid change, there...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ...

  15. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...fidelity. NEBridge GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted... °C cycling 4-base Bsal-HFv2® 37 °C Static 4-base Bsal-HFv2, 1x NEBridge Ligase MM ...They first compared 10-piece linear (the native state of the T7 genome) and circular assemblies. While...

  16. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...respectively. Chromogenic detection produces colored precipitates that permanently stain the membrane, making .... Cover the membrane in stripping solution and agitate for 5–30 minutes at room temperature. The incubation.... Cover the membrane in stripping solution and agitate for 30–45 minutes at 50 °C. Wash the membrane for...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...with BioRender.com. Add silent mutations near point mutations to create 3-base (or longer) tracts...’s all our tips for today! Each tip includes a citation to the paper it was drawn from. If we’ve piqued...

  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...the majority of the lncRNA locus, scientists can mutate the transcription start site, splice sites, exons...function (Hazan and Bester 2021; Zibitt et al. 2021). Mutating splice sites is particularly effective as it can... Transcription start site (TSS) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large...

  19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Depositing labs often provide insert sequences or annotated Genbank files that are useful for more complicated...genes with many modifications. We look for point mutations, truncations, and insertions that could compromise...compromise function. When we do find mutations, we check to see if they affect the translated amino acid. ...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...creativity and expertise of the group and yet never hesitated to reach out to the greater research community...portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus...and the School of Molecular Sciences at Arizona State University. His group carries out research in synthetic...
Showing: 341 - 360 of 718 results