We narrowed to 707 results for: ust

...To respond to environmental conditions, cells must be able to detect extracellular stimuli. One way they...of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate an...

...temperatures, so to use them in thermophiles, bacteria must be grown at lower temps. This approach only works...interesting question: why engineer a new Cas9 when you can just find one that naturally occurs? This is different...

...Publication policy seriously and appreciate the trust you’ve put in us when you choose this status for...to make sure they meet our goals, maintain your trust, and meet the community’s needs, so please let us...

... oriented in comparison to each other? The gRNAs must target different strands to create a DSB, but this...accomplished with either a PAM-in or PAM-out orientation. Just as the names imply, PAM-out designs have the PAM...

...lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The enzyme-based...at particular genomic sites when in fact they are just non-specific or indirectly interacting. The CUT...

...the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are...restriction enzymes, but you will also be able to create custom annotations and design additional experiments like...

...apparatus until I settled on using glass slides. With just the right amount of agarose, I could pour a very... volume.  So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine...

...transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means...bacterium's perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...

...priorities will help in determining a good fit. Here are just a few suggestions for determining whether an advisor...information about the PI that concerns you, don’t just brush this away. Many scientists think, “Oh, but...

...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...., 2021.   This TX-TL system is capable of robust expression of high G+C content genes, as validated...

...scientific community gets captured,” he says. “It’s not just a reagent, it’s an annotated reagent.” He’s also... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...

...didn't run down. In September we arrived in YNP just as the weather was starting to turn chilly at the...useful? It can enable applications where reagents must be stored at elevated temperatures for long time...

...particles have quite the outsized impact — and not just on the disease front. Viruses survive by hijacking...genomes have as much genome removed as possible, just to increase the viral vector’s carrying capacity...

...Depending on the cloning process, all constant regions, just the heavy chain constant regions or solely the Fc...plasmid backbone containing all constant regions, just the heavy chain constant regions or solely the Fc...

... being an editor at Science magazine. That one I just could not resist applying for. Not that I knew anything... have professional meetings, societies, networks just as do research scientists. There we meet others ...

..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...

...capable of at the same time. Barcoding libraries Just as physical barcodes help librarians track individual...libraries have improved on-target activity for more robust and accurate screens. Activation or inhibition ...

...function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis. While setting...components in the buffer used for protein purification must be helping the untagged protein enter cells. Subsequent...

...inducible promoters. Far removed from the bio-industry’s vision of ‘biofactories’, most successes in metabolic...that lacks endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity...

...in one state, but not the other). “It’s quite a robust way to make a FRET sensor with a large dynamic ...10.1074/jbc.M110.124545. (Pubmed) Lindenburg et al. Robust red FRET sensors using self-associating fluorescent...