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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 694 results for: ust

Showing: 341 - 360 of 694 results

  1. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...To respond to environmental conditions, cells must be able to detect extracellular stimuli. One way they...of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate an...

  2. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...temperatures, so to use them in thermophiles, bacteria must be grown at lower temps. This approach only works...interesting question: why engineer a new Cas9 when you can just find one that naturally occurs? This is different...

  3. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...apparatus until I settled on using glass slides. With just the right amount of agarose, I could pour a very... volume.  So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine...

  4. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means...bacterium's perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...

  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...priorities will help in determining a good fit. Here are just a few suggestions for determining whether an advisor...information about the PI that concerns you, don’t just brush this away. Many scientists think, “Oh, but...

  6. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...particles have quite the outsized impact — and not just on the disease front. Viruses survive by hijacking...genomes have as much genome removed as possible, just to increase the viral vector’s carrying capacity...

  7. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...Depending on the cloning process, all constant regions, just the heavy chain constant regions or solely the Fc...plasmid backbone containing all constant regions, just the heavy chain constant regions or solely the Fc...

  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...scientific community gets captured,” he says. “It’s not just a reagent, it’s an annotated reagent.” He’s also... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...

  10. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...., 2021.   This TX-TL system is capable of robust expression of high G+C content genes, as validated...

  11. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...

  12. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... being an editor at Science magazine. That one I just could not resist applying for. Not that I knew anything... have professional meetings, societies, networks just as do research scientists. There we meet others ...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis. While setting...components in the buffer used for protein purification must be helping the untagged protein enter cells. Subsequent...

  14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...inducible promoters. Far removed from the bio-industry’s vision of ‘biofactories’, most successes in metabolic...that lacks endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity...

  15. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...in one state, but not the other). “It’s quite a robust way to make a FRET sensor with a large dynamic ...10.1074/jbc.M110.124545. (Pubmed) Lindenburg et al. Robust red FRET sensors using self-associating fluorescent...

  16. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...institute may have a piece of equipment that is custom-built, hard to find, or inaccessible to you for..., if you don’t have a formal connection, you can just reach out to start the conversation. Many researchers...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...recombinant proteins by the pharmaceutical and biotech industries as a protein expression workhorse. This yeast...beyond K. phaffii ) worth looking into whether you’re just dipping your toes into the yeast world or have risen...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...vectors for shielding neutralizing antibodies and robust gene delivery in vivo." Biomaterials 35.26 (2014...et al. "Exosome-associated AAV2 vector mediates robust gene delivery into the murine retina upon intravitreal...

  20. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...efficiencies, additional references, etc...) and a more robust database structure was needed.  It is also difficult...Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs that ...
Showing: 341 - 360 of 694 results