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We narrowed to 707 results for: ust

Showing: 341 - 360 of 707 results
  1. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...Publication policy seriously and appreciate the trust you’ve put in us when you choose this status for...to make sure they meet our goals, maintain your trust, and meet the community’s needs, so please let us...
  2. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...To respond to environmental conditions, cells must be able to detect extracellular stimuli. One way they...of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate an...
  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... oriented in comparison to each other? The gRNAs must target different strands to create a DSB, but this...accomplished with either a PAM-in or PAM-out orientation. Just as the names imply, PAM-out designs have the PAM...
  4. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The enzyme-based...at particular genomic sites when in fact they are just non-specific or indirectly interacting. The CUT...
  5. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...temperatures, so to use them in thermophiles, bacteria must be grown at lower temps. This approach only works...interesting question: why engineer a new Cas9 when you can just find one that naturally occurs? This is different...
  6. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...apparatus until I settled on using glass slides. With just the right amount of agarose, I could pour a very... volume.  So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine...
  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means...bacterium's perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...
  8. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...priorities will help in determining a good fit. Here are just a few suggestions for determining whether an advisor...information about the PI that concerns you, don’t just brush this away. Many scientists think, “Oh, but...
  9. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    Published
    Nov. 19, 2025, 2:15 p.m.
    ...capable of at the same time. Barcoding libraries Just as physical barcodes help librarians track individual...libraries have improved on-target activity for more robust and accurate screens. Activation or inhibition ...
  10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...particles have quite the outsized impact — and not just on the disease front. Viruses survive by hijacking...genomes have as much genome removed as possible, just to increase the viral vector’s carrying capacity...
  11. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...Depending on the cloning process, all constant regions, just the heavy chain constant regions or solely the Fc...plasmid backbone containing all constant regions, just the heavy chain constant regions or solely the Fc...
  12. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...., 2021.   This TX-TL system is capable of robust expression of high G+C content genes, as validated...
  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...
  14. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... being an editor at Science magazine. That one I just could not resist applying for. Not that I knew anything... have professional meetings, societies, networks just as do research scientists. There we meet others ...
  15. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...scientific community gets captured,” he says. “It’s not just a reagent, it’s an annotated reagent.” He’s also... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...
  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis. While setting...components in the buffer used for protein purification must be helping the untagged protein enter cells. Subsequent...
  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...inducible promoters. Far removed from the bio-industry’s vision of ‘biofactories’, most successes in metabolic...that lacks endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity...
  18. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...in one state, but not the other). “It’s quite a robust way to make a FRET sensor with a large dynamic ...10.1074/jbc.M110.124545. (Pubmed) Lindenburg et al. Robust red FRET sensors using self-associating fluorescent...
Showing: 341 - 360 of 707 results