We narrowed to 904 results for: CAN

... variations in how one can gate, select, and analyze data. But now that you can read a FACS plot and follow...literature. Data reported from flow cytometry experiments can be a little challenging to understand if you’ve never...them it's helpful to have done flow first… which can send you in an endless loop. Let’s break out of the...users to analyze single cells in a population. It can be used to measure protein expression, identify rare... by the researcher, based on their controls. You can usually tell where this point is by looking at the...the cell populations as a guide  (Figure 3.) You can see that in our dot plot, we have A-B- cells, A-B...markers. There are various types of SSC and FSC that can be used for this purpose.  Pro tip! The forward scatter...

...your target cells. Poor tissue culture practices can lead to low titers and poor transductions. Cell lines...serum, other cell lines, or infected personnel and can persist undetected; unlike infections with larger...microbes such as yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching ...Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels, and ...and behavior. Signs of a mycoplasma contamination can be subtle and may include reduced saturation density... that extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the... consider concentrating your viral prep. Storage can also play a role in the success of your lentiviral...

...present the vast collection of material so that you can more easily find what you’re looking for. Whether...Vectors Fluorescent Proteins Science Careers You can use the dropdowns in the menu to further drill down...cover topics outside of these five areas and you can find what you need by field (ex: microbiology, neuroscience...or publishing, under the Other dropdown menu. You can also find information on using Addgene’s website,...new to the topic. Or, try the search bar Now, you can search for the articles you want to find without ... them? Check out the new author pages, where you can read more about the Addgenie behind the blog post...

...events in the proteomes of higher mammals (2). How can we begin to understand the importance of any of these...phosphorylation events on its activation loop, but can be made constitutively active through phosphomimetic...extensively worked with and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial... bacterial strain, TAG is an ‘open’ codon, which can be specifically recognized by the Sep-tRNA. 3. An...vector contains a mutant pSer-containing GFP that can be used as a positive control during optimization...PhosTag system (see below). This plasmid backbone can also be cloned with an ORF of interest for customized...non-phospho-incorporated proteins purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag,...

...functional Cas9 molecule can cleave the target DNA. The inducible split cas9 approach can also be used for activation...Finally, the system can be adapted to cellular screening to identify molecules that can perturb protein transport...through Addgene so you can try these incredible new tools yourself! The tags can be found in the pBluescript...that this tool can also be used to visualize translocations, genome rearrangements and cancer-associated ...shoot us an e-mail at [email protected] and maybe we can write a blog post about it! Split Cas9 system The...expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...genes are turned on or off in a given area and the can do so in a very specific, precise manner. Previously...

...particular backbone can be used to transfect a variety of mammalian cells and can be modified for viral...a versatile mammalian expression vector that you can use to express both SpCas9 and your gRNA in a variety...followed by BbsI restriction sites into which you can clone your 20 nucleotide guide sequence of choice...fused to a 3X FLAG tag at its N-terminus. This tag can be used to test for SpCas9 expression post transfection...a versatile mammalian expression vector that you can use to express both SpCas9 and your gRNA of interest... better facilitate homology directed repair. You can find all of these variants, as well as links to the...

...much as you can), form a peer mentoring group to get real feedback Write whenever you can, find supportive...Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable skills while you...Demonstrating your ability to work well with others can be done in many ways… and many labs in academia benefit...advance science faster. Help other people when you can. The colleagues you help will be future advocates... team productive. Take all the opportunities you can to be communicating about science with others. If...organizations Another excellent perspective on this topic can be found on the UK Career Site for Researchers called...

... and leaky expression can occur in cells that don’t express Cre or Flp, what can we do about it?  Test...comprised of different sequences (loxp and lox2272) and can not recombine with each other. When Cre is introduced...either end of the transgene. Since loxp and lox2272 can not recombine, this ensures that the transgene is...They found that almost all off-target expression can be attributed to spontaneous recombination between...This indicates that the amount of leaky expression can largely be attributed to the level of spontaneous...vectors will have some level of recombination that can lead to leaky expression in cells that do not express...genomes contain a recombined/functional transgene that can be expressed in cells where Cre is absent. Additionally...

... animal facility which can be expensive to gain access to routinely, and it can be especially challenging...size, nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes...10-30) of sybody candidates are sequenced for identification. All unique sybodies can be subcloned into...are small (12-15 kDa) single-domain proteins that can bind effectively to protein targets, similar to how...selection platform was designed so that any standard lab can quickly select for sybodies targeting a membrane ...regions that bind to the antigen. The Seeger lab can directly provide the three sybody libraries in the...contain serines and threonines at positions that can be randomized for creating the libraries (Zimmermann...

...and PI can provide feedback on your technical presentation, but those outside of your field can point ...Tailor your language to your audience so that they can digest what you’re saying, provide constructive criticism...determine how to tailor your presentation so that it can be understood by your audience. Use your poster as... aid, not a crutch. Even the most prepared of us can find ourselves talking to our posters, so if you ...aside to read through the list at the session so you can strategically use your time. Don’t have access to...yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-long presentations...presentations, which constrains the number of posters you can see. If you are pressed for time, limit yourself to...