... a ~20 nucleotide targeting sequence and ~75 nucleotide scaffold sequence) is consistent between plants...contain a plant pol III promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos...is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus, and several...the specifics - promoters used, precise protein sequences or domains, and methods of delivery - the underlying...

...repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used ...plant source), by tuning the length of 5’ leader sequences of a given tRNA to achieve the highest efficiencies...

...transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery... genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed. Gal4...multiple genes under the control of UAS enhancer sequences. For example, if you discovered five new genes...

...protocol before performing single-cell RNA sequencing. The sequencing results covered TFs representing 92% ...average of 206 cells per TF ORF. From this large sequencing endeavor, a map of possible TF-induced cellular...

... (bEBPs) at a sequence upstream of the promoter called the upstream activating sequence (UAS). The Wang...phenotype can be identified by next-generation sequencing of the genomic DNA. However, some cell types ...

.... Piecing together multiple PCR products using sequence homology is also common. Overlap extension PCR... to splice together two PCR amplicons based on sequence homology, and the subsequent product is then digested...fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria...

... strand at each replication fork. Termination sequences present in the chromosomal DNA ensure that the...pSC101 itself. RepA binds to directly repeated sequences called iterons located near the ori and directly...ColIb-P9 plasmid, is complementary to part of the sequence forming this pseudoknot, so its binding prevents...replication may differ in the specialized proteins or sequences necessary for initiation or the mechanisms that...

...platform is compatible with enrichment, single-cell sequencing, and optical pooled screens in human cells. Addgene... improve the specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence...alone or in combination with miR124T targeting sequences, provides a simple yet valuable tool for microglia... REVeRT to functionally reconstituting coding sequences for a range of applications, including CRISPR ...

...inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon on pTargetF (Jiang... this system, you’d first introduce your sgRNA sequence into the pTargetF construct and then introduce... process begins with the addition of the sgRNA sequence in pEcgRNA. Next both plasmids are transformed...pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ccdB gene containing BsaI...

...longer stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. ...variants with optimal function. High-throughput DNA sequencing is then used to measure the frequency of each...more codons you test in one library, the more sequencing reads you will need. See Fowler et al for more...