We narrowed to 432 results for: sequence

...use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately, the vast majority... majority of antibodies are supplied with no sequence information, leading to problems with reproducibility...affinity reagent screening, and next-generation sequencing of hybridomas have opened the door for a new ...

... editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA sequence targeted by the...complex, promoting its interaction with the degron sequence and ultimately its degradation. While other eukaryotes...

...sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013). Modifying the CRISPRi system... will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional regulator...Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152...

...please contact us. Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

... a genetic sequence and integrate it into any DNA sequence containing the 38-50 bp sequence recognized...they optimized the specific attachment sites via sequence engineering, enabling even more efficient integration...

... effective, ivTRT may be more challenging for sequences with complex secondary structure. Today, multiple.... Miura et al. (2018) suggest using a 50-80 bp sequence around the desired insertion site to search for...C-terminal insertion with Easi-CRISPR. Potential guide sequences (arrows) and their PAM (bold highlighted text)... knock-in design may naturally remove the gRNA sequence or place it far from the PAM; if not, you can ...guide. This design also separates the gRNA target sequences from their PAMs, preventing re-cutting after editing...

...the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF. Negative...protein is present, but cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein... (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In the presence...binds to promoters containing putative operator sequences (CuO) (linked in groups of 6), allowing transcription...which activate their respective UAS and lexAop sequences but are also regulated by QS and quinic acid. ...

... a ~20 nucleotide targeting sequence and ~75 nucleotide scaffold sequence) is consistent between plants...contain a plant pol III promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos...is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus, and several...the specifics - promoters used, precise protein sequences or domains, and methods of delivery - the underlying...

...protocol before performing single-cell RNA sequencing. The sequencing results covered TFs representing 92% ...average of 206 cells per TF ORF. From this large sequencing endeavor, a map of possible TF-induced cellular...

...transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery... genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed. Gal4...multiple genes under the control of UAS enhancer sequences. For example, if you discovered five new genes...