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  1. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...plasmid or simple template, there is very little chance for mis-priming, so you can use a pretty wide range...

  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...integration causing mutations and gene expression changes elsewhere in the genome. Second, expression is ...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...becomes hard to accurately execute. However, these challenges can be circumvented by generating individual ...

  6. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Special thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this...

  15. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...rapidly excite GFP. As a result of the different mechanisms to produce photons, chemiluminescent reporters...

  20. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...Leeson Multiplexed images in live cells remains a challenge due to multiple factors, including limitations...

  22. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...plot showing a single color control for the APC channel. B) and C) A flow plot showing an APC single color...

  25. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...pUC19 Let’s begin at the beginning. The well-characterized bacterial lac operon contains a gene called ...

  32. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...29608282. PubMed Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad...

  34. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

  35. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

  36. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...26814968. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

  37. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...small size of the polyhistidine tag minimizes the chance of the tag disrupting normal protein function, ...
Showing: 721 - 760 of 793 results