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Showing: 361 - 380 of 602 results

  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...through the stomata into the abaxial site (lower surface) of the leaf. This is a simple and easy technique...Mortensen et al., 2019): Leaves or whole plants are placed in an Agrobacterium solution and a vacuum is applied...

  3. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...are 27 incompatibility (Inc) groups in the Enterobacteriaceae family alone, and this is expanding as we...expelled antibiotic resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman...

  4. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and vary in shape and structure. Their porous surface provides a large area for binding, but antibodies... than agarose beads. Proteins conjugate on the surface of magnetic beads, and are therefore completely...

  5. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...editors Excises G, T, or C, respectively, to be replaced with another base. References Chen, L., Hong...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...commercial antibodies can be tethered on the viral surface, expanding the range of targetable cell types. ...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

  7. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...without disrupting plasmid representation. A good place to start is with a protocol for a library of similar...Moderated by the Feng Zhang lab, this is a useful place to ask experimental questions.  Practical Considerations...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...struggling to understand how the researchers decided to place their gates on the plots! Flow data is messy, so...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  10. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... or the statistical variation is calculated and placed in the legend. Widely reported difficulties in ...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

  12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...structural complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser...through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse CD11b-BV510), which is...

  13. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ..., their makeup (in terms of nationality, gender, race, etc), or their career outcomes. Some of the more...their mentorship with the same critical eye they place on others within their labs - as a sort of over-...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental...inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80 under different promoters and create...

  16. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

  17. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy: class to type to.... However, knowing the basic diversity and the surface-level function of each type could lead you to new...

  18. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves...plasma membrane and can be detected through a cell surface label. The viral vector also encodes for eGFP, ...

  20. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...
Showing: 361 - 380 of 602 results