...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...budgets, “a large percentage of survey respondents indicated that cost was a significant barrier to ELN adoption...to digital for example): “… 99% of respondents indicated that ease of use would influence their ELN choice...that their research ‘doesn’t fit neatly into one category;”. The study also showed that It is important ...to keep all their data in one place and avoid scattering intellectual property across various software...collaborative functionalities include comments, notifications, sharing files, assigning team members to certain...

...variety of species, cell types, and research applications. Labeling neurons with Brainbow The Lichtman... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...not fluorescent. Instead, they incorporate a truncated, non-fluorescent form of YFP as the default state...recombination can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice, some neurons expressed...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent protein genes...

...studying genetic modification for their ease of manipulation and their ability to be replicated. Because E...., 2004). Additionally, breakthroughs in gene modification tools and methods such as CRISPR have vastly...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef... bacteria with superior performance. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-04899...

...that the oversized AAV genomes are fragmented or truncated when packaged and then following transduction,...and may need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller...transgene to help prevent the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008...design, donor A may be capable of expressing a truncated protein. This can be avoided by excluding a stop...JS (2011) Gene Therapy of mdx Mice With Large Truncated Dystrophins Generated by Recombination Using rAAV6...Improved dual AAV vectors with reduced expression of truncated proteins are safe and effective in the retina ...

...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...interest by recording DNA abundance throughout the amplification process, rather than just at the end as in standard...experiments allowed researchers to identify the location of genes. With this knowledge, researchers were...was added and when. Compiling the identity and location of a given nucleotide, a researcher (or a simple...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

...and easily navigate to educational resources, tools, collections in our catalog, and the Addgene Help ...search bar. And if you’re in the mood to browse our catalog, you can use the links under the search box to ...scrolling up and down the page.  Figure 3: The Education & Tools menu Further down What happens if you ... mission, links to our deposit, request, and educational resources page, and previews for our three most...

...speak for you (and you for them) Communication Being able to communicate effectively is part of what makes...you can. The colleagues you help will be future advocates and references. Collaborate with another lab,...productive. Take all the opportunities you can to be communicating about science with others. If opportunities ... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...

...results, modifications, and inventions created by the Recipient Right to file patent applications on modifications... about intellectual property rights, pending publications, and commercial use often come up. An MTA can...trials Acknowledgement in recipient’s future publications relating to the material Limited liability...

...channelrhodopsin-2 (ChR2), a blue light-activated cation channel, from the archaebacteria Chlamydomonas ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...2003). Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proceedings of the ...bidirectional dual-color control of neurons. Nature Communications, 12(1), 1–20. https://doi.org/10.1038/s41467...

...site, or when using chemogenetic tools, where modification of cellular activity outside of your experiment.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...comparative analysis of constitutive promoters located in adeno-associated viral vectors. PLoS ONE, 9(...Approaches and Further Perspectives of AAV Tropism Modification. Pharmaceutics, 13(5), 750. https://doi.org/...Retrograde Serotype Viral Vectors 101: Viral Applications...

...honestly evaluate your own style of work and communication before making this important decision. I’ll ...Prepare by going sleuthing on the lab website, publication records, LinkedIn, ResearchGate or Twitter. Be...the appropriate first authorship position on a publication of her project and made another lab member first...– including failure to acknowledge good work, allocation of meaningless tasks, removal of responsibility...connections such as serving on Boards? Does the publication record show evidence of industry collaboration...skills guide Learn about careers in science communication Read posts on mentoring for scientists Resources...

...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...of the piggyBac™  transposase/transposon gene modification system and the scarless, Footprint-Free™ removal...technology that may be used for a variety of research applications, including making transgenic cell lines and ...PMCID: PMC2567143. 6. S. Radecke, F. Radecke, T. Cathomen, and K. Schwarz, “Zinc-finger nuclease-induced...oligodeoxynucleotides: wanted and unwanted target locus modifications.,” Mol. Ther., vol. 18, no. 4, pp. 743–53, Apr...Addgene.org Find Vectors with the piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit...

...with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently...shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop a searchable...The efficacy of gRNAs is impacted by the target location, target sequence, and model system. Even if a ...and final experimental outcome in the original publication. As you develop and confirm new gRNAs, please...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein...

...mostly because of this article: “A systematic identification and analysis of scientists on Twitter.” This...from the United States Standard Occupational Classification System  and Wikipedia. 2. Social and computer...: Portion of Tweets with Scientific Domains, Categorized by Discipline. Biologists, neuroscientists, and... Y., & Sugimoto, C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos...Scientists 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Why Scientists...

...working in your chosen field. This cooperative education experience (co-op) is what has brought me, a second... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...for the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...currently working on projects for the Science Communication function at Addgene. Working a 9-5 As it turned...

...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... same Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their...simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated for the crucial role of basic research in the ... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...light. As the researchers described in Nature Communications last year, “The capability to control spectral...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670...fluorescence is because the light becomes very scattered,” Verkhusha explained. Ultrasound wavelengths,...hand, are larger than the size of a cell, so they scatter much less. As a result, ultrasound enables higher...

... difficult to eradicate. You can suppress the growth of mycoplasma but cannot eradicate it completely....Contamination! Tissue culture media often have indicators included in the media that turns yellow from ...important to prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects...the first person to face this kind of problem. Educate others when you can and help them understand the...Cc, Drexler Hg (2002) Comparative Antibiotic Eradication of Mycoplasma Infections from Continuous Cell...

...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...most scientific collaborations fall within this category. Unlike a process team, you don’t necessarily ...functioning (or malfunctioning). Clear lines of communication Few people actually like meetings, but run well...are helpful, they are certainly not the only communication options. Addgene, along with an increasing number...desks away or on the other side of the world, communication platforms like Slack make teamwork easier. Trust...excellent leader, and perfectly clear lines of communication, but if teammates don’t trust each other, none...