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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: CaS;

Showing: 361 - 380 of 610 results

  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...of having all the pages in a Curricula Vitae to showcase your publications, academic performances, and ...related to the job you are applying for, in which case talk about your research, as an understanding of...

  2. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...should be interesting; however, this should (in most cases) not be the driving factor. The following questions...head for a career in pharma or biotech, this is one case where the topic of your research can make your transition...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...following exposure to a brief heat shock. In the case of Hsp70, the heat shock releases heat shock factor...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...validate your antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite ...abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion can preserve more...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

  8. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...during in vivo research and the CARE guidelines for case reports are two sets of guidelines frequently used...manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers...

  9. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... the excitation power (Grünwald et al, 2008) occasionally and certainly after a set-up has been changed...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...given threshold within the cell, as is often the case upon viral infection. Endogenously produced dsRNA...the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  11. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...primary-conjugated) or indirect (secondary) methods. In those cases, remember that a secondary should be used when signal...you still have a number of options. If this is the case, you can narrow them down in a variety of ways. ...

  13. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... that expresses well, then probably not. In this case, take a look at the secondary antibodies that your...to probe multiple targets in parallel. In these cases, it is critical that users choose antibodies that...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

  17. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...

  18. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation limiting...molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...

  19. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai incident, and ...schools, community events, churches or mosques. On occasion, we also get interviewed for print media, radio...
Showing: 361 - 380 of 610 results