Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 801 results for: Cre

Showing: 361 - 380 of 801 results

  1. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing... the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

  2. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...product and development teams have worked hard in increasing and improving accessibility on our website.  ... In fact, one of our team goals in 2023 was to increase accessibility to our website through the use of...describe images in online spaces. Alt text is used by screen readers to provide visually impaired visitors with...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

  3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain the expected...of plates will have select samples prepared and screened by NGS to spot check the samples, the rest will...are requested by scientists, will immediately be screened by our QC scientists to confirm the plasmids contain...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

  4. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Welcome to our deep dive series, which aims to increase your understanding and technical proficiency with...temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from single-stranded...finally, drop once more during a final temperature increase. If there is a single amplicon, there should only...c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019...can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes Whatever...

  5. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...ions flow into the neuron and causes it to fire. Created with BioRender.com. To make channelrhodopsin-based...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model...fiber-optic cable allows blue light to reach the brain. Created with BioRender.com. In the first experiment...neurons in the VTA, this should propel the mice to increase their nose-poking behavior. Strong nose-poking...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...it, it just reflects how useful the reagents we created are for the community. More than 100 labs worldwide...need for high-quality service, open data, and increased reproducibility of materials. In March 2022, we... supported by an NIH BRAIN Initiative. We also created a growing library of antibody educational resources...York, Calico Life Sciences. Most of all, we’re incredibly thankful for all the scientists who have joined...

  7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...Voigt: The big promise is orthogonality. You can create an almost limitless number of DNA binding proteins...fundamental. We built a NOR gate because they are used to create any program you can imagine. The Apollo 11 mission...so that genetic designs of that scale could be created. Someone could take on an engineering project including...enough hardware to control hundreds of genes. We’ve created enough regulatory elements, CRISPRs, repressors...

  8. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...OpenWorm OpenWorm is an open source project that is creating the “first virtual organism in a computer.” This...and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system...reagent toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each contains...2016). Using SapTrap, this CRISPR plasmid can be created in a single tube. You can also find additional ...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...observe the effects. Luckily for us, CRISPR is an incredibly versatile system that can be tweaked to target...pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because very small...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...of Science Writers How to create a successful science blog  5) Get creative – Bring your knacks into ... live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try your hand ...Science communication is my way of living out my creative side. My newest, exciting endeavor is starting...

  11. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...suspected a data-driven approach might allow for an increase in the complexity. In 2018, they published a paper...conditions available in DAD tools While DAD made an incredibly complex reaction possible, NEB also optimized...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...Ultimately, data-driven Golden Gate Assembly greatly increases the potential complexity allowable by a favored...

  12. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... you typically would at a microbiology meeting. Create an outline: Write key bullet points for what you...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...off as a broken record, remember the outline you created for yourself. Hit the key bullet points and elaborate...will help you to provide an on-topic answer by creating time to organize your thoughts. It’s likely you...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...templates in somatic cells of adult animals in order to create animal models for particular cancer. That’s exactly...the Memorial Sloan Kettering Cancer Center did to create a model of Eml4–Alk-driven lung cancer (Maddalo... Addgene and could be useful to labs wishing to create this kind of animal models. Start using adenoviral...Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers M, Maggio I, Henriques SFD...

  14. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... you and your goals. Making a List of Schools Creating a list of schools is one of the first steps in...submitting your application. I found this step to be incredibly useful, as it gives a more comprehensive idea...advisor’s research interests which can be helpful in creating your application to that program. An email to ...simply not feasible. Through the virtual systems created and promoted during COVID-19, online meetings with...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...Hayward et al., 2024). The fluorescence intensity decreased whenever the magnet approached the sample (by ... and the team set up a directed evolution and screening experiment with three of the proteins that had...several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no... suggest she deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... we performed high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled ...cell contexts. Figure 1: High-throughput screening of pegRNA efficiency and building prediction tools...sequence-based prediction models from self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights...your target. Figure 3: High-throughput screening of the effect of chromatin on prime editing efficiency...

  17. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...methods. While techniques such as  blue-white screening require X-gal, an expensive exogenously-added ...chromoproteins don’t require other substrates for screening. Thus chromoproteins can be used as markers in... used the amilCP and tsPurple chromoproteins to create negative selection markers for allelic exchange...transfer (FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...demonstrated to result in decreased mismatch tolerance and, consequently, increased specificity relative to...more stringent targeting rules that result in a decrease in available target sites. Regulating gene expression...focused advancing scientific discovery through the creation of innovative technologies. To date his group ...

  19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Cas9 should be aware of the potential hazards of creating “gene drives” capable of spreading through wild...does making transgenic organisms with Cas9 risk creating a gene drive? The simplest way to make a gene ...appropriate flanking homology into a germline cell can create an RNA-guided gene drive. In principle, this event...Consequently, the best way to avoid accidentally creating a gene drive is also the easiest: don't use a ...

  20. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...White House as a Champion of Change, a program created as part of President Barack Obama's Winning the...DNA. We thought these were so much fun and so incredibly useful that we wanted to make them free to use... the invention of genetic engineering. What's incredible is the bioeconomy is already pretty substantial...product – equal to mining. There is an absolutely incredible hidden story here. The parts we are releasing...
Showing: 361 - 380 of 801 results