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We narrowed to 694 results for: EED

Showing: 361 - 380 of 694 results

  1. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...writing, and networking Practice your English if you need to, go to ESL classes Practice your presentation... can), form a peer mentoring group to get real feedback Write whenever you can, find supportive editors...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ... 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making...’s easy to make your own competent cells. If you need a high efficiency of transformation (for example...

  3. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...designed a Golden Gate system based on the daily needs of an advanced molecular plant science lab.” Introducing...distinct sites where the enzyme will cut. You only need a single enzyme to cut fragments that are very different...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...other fluorescent proteins for your imaging needs. If you need an FP refresher, see our FP blog posts.   ...

  5. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...Tweeted comments: “A good mentor has your goals/needs/situation in mind, rather than (or as well as) their...and communicates clearly and openly Gives honest feedback without ever being demeaning Does not micromanage...

  7. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...boundaries. To be able to beat this virus, we will need to do the same with science,” said Guillaume Bourque...Human Genetics at McGill University. “There is a need to pool and maximize resources nationally and internationally...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...Transparency, both in what works and in what doesn’t, and feedback are vital components of building both the community...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...

  9. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...Addgene’s work, mission, and values. I welcome feedback and commentary from our diverse community, which...success, and for believing, as we do, that sharing speeds science.  This year, Chonnettia will be sharing...

  11. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...help you. If the organization is large, you will need to be more directed and target someone in a specific...much better chance at an interview –but you still need to nail the job yourself.   See the links in the...

  12. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...interdisciplinary.   3. “There are many different ways to succeed – as loner or gregarious;  dictator or laissez-...ways to have a large positive impact in society. We need much, much more science and engineering (STEM) expertise...

  13. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ... Addgene has no investors or stock. All of our proceeds go into running the repository and serving scientists... about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen...

  14. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... biosensors When choosing a biosensor, you first need to decide the best way to track or measure the process...experiment. For example, does the fluorescent tag need to be pH stable or withstand a particular solute...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Invasiveness Both optogenetic and chemogenetic tools need to be delivered to cells, commonly through viral...Acknowledgements We thank the Bryan Roth lab for feedback on this article and sharing their expertise with...

  18. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... often used interchangeably with the term sgRNA. Seed sequence: The 8-10 bases on the 3’ end of an RNA...CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

  19. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...cloning and expression from plasmids, due to only needing one open reading frame Ability to access unique...to producing single-domain antibodies without the need for a species that naturally produces heavy-chain...

  20. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... easier for you to find and use the plasmids you need. Here’s what you’ll find in this article: CRISPR-based...catalytic activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was...
Showing: 361 - 380 of 694 results