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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: HA

Showing: 361 - 380 of 738 results

  1. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify...in human pluripotent stem cells, a cell type that has been difficult to modify in the past.   Figure...

  2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...depositing lab. If your vector is large (>10 kb) or has a low copy number, try following protocols for low...easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that will...

  3. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... A practical guide by Paula J. Silvia  This book has tips for how to write a lot as an academic. If you...your Mendeley library in Microsoft Word. Mendeley has a web and desktop version that works with Windows...

  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...

  5. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ... an assistant professor at Kyushu University. He has a particular interest in the development of sensory...Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects of...

  7. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ... have been spent within a lovable community that has allowed me to expand my knowledge more than ever ... had opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want...

  8. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...cells, but since the majority of the viral genome has been swapped for alternative genetic cargo, they ... there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for...

  9. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...library screen; instead I thought they would focus on “Has this person been consistent throughout their career...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...prokaryotes; the ultimate victor will be the species who has the strongest will to survive. Genome engineering...biological and translative technologies. Addgene has empowered researchers to harness previous experimental...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... beta-barrel structure. Although the beta barrel has no strong affinity for other proteins, it may still...modifications. Despite these potential issues, GFP has been used successfully in countless fusion proteins...localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization (Shaner et ...

  12. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...track single cell dynamics at the organism level has been made possible by tools that allow cells to become... such as neurons in the fly visual system, which has been difficult to study previously (Hadjieconomou...of cells in the intestinal crypt, and since then has become a powerful tool for understanding cell proliferation...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...with the macromolecules it is added to. Biotin also has an exposed side chain that can be easily manipulated...coated with avidin. The biotin-avidin interaction has also been adapted for use in immunohistochemistry... either the N or C terminus of the protein. BirA has been shown to effectively biotinylate AviTag expressing...

  14. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%. Your experiment...Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization which...where only DNA sites bound by Cas are sequenced, has been tested with some success. The major con is that...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity. All of...could be extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment ...engineering other enzymes. Conclusion We hope this post has illuminated some of the many tools available for ...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most..._block %}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid..., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...

  17. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... editors be close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at...or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with... abasic site. The same protein (or its homologs) has more than one name. Indel Insertion/deletion A ...

  18. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...means persuading an employer that your past work has prepared you to contribute at a high level to their...rewarding. A truly passionate community of scientists has formed around science communication, including the...

  19. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often than not...these tools and to tell us how useful the service has been for their experiments. This helped reinforce...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...
Showing: 361 - 380 of 738 results