We narrowed to 743 results for: HA

... have been spent within a lovable community that has allowed me to expand my knowledge more than ever ... had opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want...

...cells, but since the majority of the viral genome has been swapped for alternative genetic cargo, they ... there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...

...requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify...in human pluripotent stem cells, a cell type that has been difficult to modify in the past.   Figure...

...depositing lab. If your vector is large (>10 kb) or has a low copy number, try following protocols for low...easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that will...

... A practical guide by Paula J. Silvia  This book has tips for how to write a lot as an academic. If you...your Mendeley library in Microsoft Word. Mendeley has a web and desktop version that works with Windows...

... an assistant professor at Kyushu University. He has a particular interest in the development of sensory...Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects of...

... to objects that exist online, but this approach has been adapted for the identification of physical materials...Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier...

...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...

...TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So how does...PCR products. Keep in mind that Taq polymerase has an error rate of about 1 in 3,500 bases. Typically...

...library screen; instead I thought they would focus on “Has this person been consistent throughout their career...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ...

...prokaryotes; the ultimate victor will be the species who has the strongest will to survive. Genome engineering...biological and translative technologies. Addgene has empowered researchers to harness previous experimental...

... editors be close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at...or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with... abasic site. The same protein (or its homologs) has more than one name. Indel Insertion/deletion A ...

...localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity. All of...could be extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment ...engineering other enzymes. Conclusion We hope this post has illuminated some of the many tools available for ...

... beta-barrel structure. Although the beta barrel has no strong affinity for other proteins, it may still...modifications. Despite these potential issues, GFP has been used successfully in countless fusion proteins...localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization (Shaner et ...

...track single cell dynamics at the organism level has been made possible by tools that allow cells to become... such as neurons in the fly visual system, which has been difficult to study previously (Hadjieconomou...of cells in the intestinal crypt, and since then has become a powerful tool for understanding cell proliferation...

...personalized genetic medicine (Musunuru et al., 2025). Much has been made of the speed with which KJ’s successful...acceptable in every case, however, and recent work has already been published on improving base editing ...R. M., Pacesa, M., Smith, B. K., Christie, K. A., Ha, L. L., Meis, R. J., Clark, A. B., Spinner, A. D....

...goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most..._block %}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid..., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...

...specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%. Your experiment...Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization which...where only DNA sites bound by Cas are sequenced, has been tested with some success. The major con is that...

...with the macromolecules it is added to. Biotin also has an exposed side chain that can be easily manipulated...coated with avidin. The biotin-avidin interaction has also been adapted for use in immunohistochemistry... either the N or C terminus of the protein. BirA has been shown to effectively biotinylate AviTag expressing...