Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 791 results for: Kin

Showing: 361 - 380 of 791 results

  1. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...eBook collection is a handy resource for anyone looking to use antibody-based applications in their research...: What is an Antibody” quite useful. If you’re looking to design your first flow cytometry experiment,...the Antibody 101 eBook is a great place to start looking for answers.    We hope you find the latest addition...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying...pressure-test your FRET assay to make sure it is working? This article addresses these questions, which ...s best to consider these cases separately when talking about the tools that are available. Let’s consider...Denis V. Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." ...

  3. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...Fly-related Issues in the Lab A significant drawback of working with fruit flies is their ability to escape their...experienced the minor yet consistent annoyance of walking into a fly lab and finding ourselves accosted by...system in a video filmed at Addgene:     If you're working in fly research, I hope you find this new technology...

  4. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...viral vector specialist, Rebecca. They will be working with Andrew, the head of our scientific support...Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and Accessible Scientific..., Instagram, and Bluesky.   Finally, if you're looking to brush up on your conference skills, check out...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...   Unbound Cas9 Now that we know what we’re looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus... the absence of guide RNA, preventing Cas9 from making off-target cuts.   Pro tip! X-ray crystallography...region between the two (Jiang & Doudna, 2017). By linking the two nuclease domains, Cas9 ensures both are...biology can help you visualize the system you’re working with and better understand how to manipulate it...

  6. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for... So how have scientists solved this problem? By taking advantage of repair pathways within the cell — ...mammalian cells Researchers in the Xueli Zhang lab were making similar observations around the same time. (The...

  7. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...If you’re new to plasmids, we highly recommend checking out the video). Tom has made many more Science...sciencewithtom.com. Many thanks to Tom McFadden for taking the time to sit down and talk with us. Tom McFadden...McFadden is a YouTuber and science educator looking for creative ways to implement the next generation science...

  9. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...common techniques and concepts. But what if you’re looking for information beyond the 101? That’s where our...our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions...check out our Instagram all summer long to see what kind of shenanigans we get into!  We hope you are as ...

  10. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ... used to thinking about the downstream results of actions. I’m also pretty adept at thinking about risks...was in the early 80s, and biotech was really just taking off. I found out that patent attorneys with a biology...

  11. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...s not the virus itself that kills you, but the cytokine storm it ultimately sets off.  The virus works...only viral protein present on the virion surface – making it critical for viral attachment and fusion. Once...attachment and fusion. Recommended Reading For those looking to delve deeper or stay tuned, there are plenty...

  12. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... expand reagent sharing even broader and began working with tech transfer offices to make reagents available...Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it...this year and along with it, wrote these tips for working with RNA and extracting RNA without a kit. 3. ...

  13. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...2018 Microbe meeting. Soon after I arrived, I was looking through a paper-based, affordable, and portable...fascinated by the world that reveals itself when looking down a paper thin and very portable microscope....the scientist in everyone - everywhere! From ‘looking at’ to ‘listening to’ microbes But seeing microbes...

  14. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...CRISPR For my editor’s choice picks this month, I’m picking two posts that describe methods to make single ...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as each one has something...

  15. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...this interest in the ways science is shared and in making it easier to share, we take special note as the...bump in citations for their trouble, even when all kinds of other factors, including journal impact factor...Way to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As...

  16. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038/srep11771. PubMed. McKinney SA, Murphy... the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative...

  17. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs details the use of CRISPR...biallelic deletion clones:    Like the video? The Orkin and Bauer lab's related paper describes their methods...Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received his BSE in Bioengineering...

  18. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...of lab BBQ parties - despite BBQs being wishful thinking in England. The Cepko Lab throws going away parties...celebrate each other's accomplishments? Any tips on making time to relax and/or enjoy time away from the bench... Dr. George Church (Harvard Medical School) for taking the time to speak with us throughout this series...

  19. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...use the search bar to quickly find what you’re looking for. Tip 3: Share protocols and spreadsheets with... knowing the information is backed up and edit tracking will help you identify when others have added ...

  20. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...nLightR greatly expand the toolbox for researchers looking to study norepinephrine, the Patriarchi lab continues...Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making protein folding accessible to all. Nature Methods...Technique is Best for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene....
Showing: 361 - 380 of 791 results