We narrowed to 405 results for: Lif;

1. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...attention! They isolated DNA from the sample, amplified the CRISPR locus from the bacterial genome, and...

2. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...edited and unedited (control) populations is PCR-amplified and Sanger sequenced. ICE compares these sequence...

3. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   Published

   Nov. 14, 2017, 2 p.m.

   ...professional development Professional development should be life long, and doesn’t end after you get your first new...

4. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ..., and feels privileged to be part of the vibrant life sciences research and commercialization community...

5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...genes that couple host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences...

6. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...Larry Gold, developed methods for selecting and amplifying RNA sequences that could bind very specifically...

7. How to Write a Scientific Review Article

   Type

   Blog Post

   Published

   Feb. 16, 2017, 3:30 p.m.

   ...that this is just a review article and not your life’s work: so remember that done is better than perfect...

8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...PubMed Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific...

9. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ... proteins in heterologous hosts. If you simply amplify a gene of interest from the human genome, for instance...

10. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...vertebrate models such as mice, which are less prolific and more expensive per individual to maintain....

11. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

12. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...

13. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...requirements of the FLSA. In the University of California system, the postdoc union has negotiated a raise...

14. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

15. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131 (DE3...

16. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...expression mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, ...

17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...blot, and depending too much on normalization can amplify other errors in experimental design and gel quantification...

18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    .... When he's not in the lab, Paul enjoys weight lifting, sailing, and reading.   More resources on the...

19. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ... a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed. Regulation of DNA double-strand...

20. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ... be the most controversial, so I would like to qualify it. If you are attempting to resolve conflicts ...