We narrowed to 457 results for: PAC

1. Using CRISPR/Cas9 to Edit Disease Out of the Genome

   Type

   Blog Post

   Published

   June 23, 2022, 1:15 p.m.

   ... difficult to use larger enzymes. To reduce the impact of off-target mutations, scientists are using sgRNA...

2. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...biology where questions of ethics can have profound impacts on the development of applications with the potential...

3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ... Targeting three dCas9 molecules to a precisely spaced region allowed sequence replacement rates of approximately...

4. How to Lead a Great Meeting

   Type

   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ... include every person who could potentially be impacted by a project. Instead, invite the experts on the...

5. Advice for Scientists Starting a Lab

   Type

   Blog Post

   Published

   April 29, 2014, 1:43 p.m.

   ... environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his...

6. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...Distributing hardware via globally established makerspace networks and training users via workshops, seminars...

7. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...genes are assayed for their role in a particular capacity. Drug screens, on the other hand, are often performed...

8. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain...

9. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ...information, however, did not improve at the same pace as the quantity and quality of the information. ...

10. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ... opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized...

11. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...The goal of this field is to functionally map the impact of cancer variants at scale. Check it out: Progress...

12. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...of a nucleus as “linear” is a misnomer. The DNA packed into our chromosomes is very much 3-dimensional...

13. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the immune system,...

14. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture...

15. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ... General Auditor at Tufts Health Plan. In that capacity he leads the Internal Audit function and is responsible...

16. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...ve: 1) convinced you social media can positively impact your career, 2) given you tips for using various...

17. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...

18. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...believes that this system could potentially be packaged into an AAV system. dCas9-SSAP will make the editing...

19. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ... the adenine in AACNNNNNNGTGC or GCACNNNNNNGTT Impact on cloning and digests Although not all prokaryotic...

20. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...gain-of-function screening when combined with a compact pooled sgRNA library. Our SAM-mediated screens ...