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We narrowed to 621 results for: SCR

Showing: 361 - 380 of 621 results
  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human scFV...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...
  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...
  3. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...
  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...
  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...
  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...optical sectioning of a specimen is based on discriminating the out-of-focus reflected light by using a...
  7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...well in plants and give similar stability to transcripts as the ones that people use all the time. Now... Marillonnet. ACS Synth. Biol., Just Accepted Manuscript, DOI: 10.1021/sb4001504. Journal. A modular cloning...
  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...
  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...
  10. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...
  11. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...
  12. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...
  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...s lab performed a series of positive selection screens in bacteria to identify mutants of S. pyogenes ...sequences (Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with...
  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... and inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...
  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative... of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition and integration...
  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...
  17. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...
Showing: 361 - 380 of 621 results