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Showing: 361 - 380 of 606 results

  1. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...the results of studies on reducing antibiotic prescriptions for respiratory tract infections. The video ...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...

  3. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

  4. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...

  6. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...customers, internal resources and databases are scrutinized, and this information is enriched with your technical...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative... of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition and integration...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems. The original pCas...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...s lab performed a series of positive selection screens in bacteria to identify mutants of S. pyogenes ...sequences (Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with...

  12. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... and inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...

  13. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  14. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...act as a feedback mechanism to reduce further transcription. Replication is also regulated by the balance...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... lines you are using, it is best to start from scratch. Obtain a vial of the desired cell line from a ...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

  16. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

  17. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... from a gene synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

  18. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...unicellular organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment...

  19. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive) conflict...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...
Showing: 361 - 380 of 606 results