We narrowed to 499 results for: SHA
-
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....your notebook. This will make it easier for you to share plasmid information with colleagues, or explain ...
-
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....skillset and interests. As such, I want to be able to share more about this nontraditional career path for others... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the ... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....multiple steps to avoid using multiple fragments that share homology in the same reaction. In these cases, the... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School. More resources... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....plasmids could be used by other labs to preserve and share their own collections. Why Recombinant Antibodies... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....freeze-thaw-freeze cycle before, or otherwise been subject to mishandling? Is the experiment you’re planning to run with... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent Biosensors for Neurotransmission... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....The spectra for mOrange2 (scaffold of OCaMP) are shaded in orange. Data from FPbase.org. Scientists... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....more. Which luciferase and assay have you used? Share your tips below! This post was originally written... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....align it with these new goals, which I’d like to share below. Common pitfalls in the transition (what I... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....Lentivirus Lentivirus is a type of retrovirus and shares many common features with gamma-retrovirus including... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....less likely that this amino acid sequence will be shared across other proteins. Conversely, polyclonal antibodies... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....perfected processes to curate the data our depositors share and enrich it with sequencing data; as a result,... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....):337-47. PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....just one reaction. Using other methods, such “BAC shaving” would require multiple steps. One potential pitfall... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....It’s a simple solution. It would be silly not to share it as soon as possible. Addgene: How much interest... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog PostPublishedAug. 7, 2014, 1:58 p.m....post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....make resources easier to identify, find back, and share. Depending on the type of manuscript you are writing... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....-translationally modify proteins yet they still share many of the same technical advantages that come ...