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Showing: 361 - 380 of 520 results

  4. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... Mello labs also identified several experimental set ups that could improve CRISPR efficiency in C. elegans...introduced. Cre-mediated excision of the selective cassette minimizes the sequence added, leaving a 34 bp ..., however the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple...Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR...

  6. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... easier to automate and use in high-throughput settings, but when it comes to low-throughput applications...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...reactions with relatively little work after the initial setup. (Addgene actually uses some of these — if you’re...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...optimize your activation protocol for your particular set up. Applications of photoactivatable fluorescent ...and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular Cloning System.” PLOS... chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those looking to express ...different promoters, terminators, enhancers, and cross-cassette interactions in gene expression, and to generate...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with four being ...including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG cassette.    Find gRNA multiplexing...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...optimization before the field begins to converge on a set of standard tools. Additionally, aspiring genetic...

  12. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...You’ll want to select a pre-stained or unstained set of standards that covers a size range appropriate...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...AAV.CAP-B22 CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 ... of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis...

  14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed during...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population...cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...effector is expressed. In cells where the stop cassette remains, the effector is not expressed and the...attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis Delta ... Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...

  15. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...decorative" title="Check icon" %} Just bacteria {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Excellent No Bacteria and eukaryotes (G418) {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...purpose="decorative" title="Check icon" %} {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Check icon" %} Carbenicillin Excellent {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... switch conditional knock-in cassette. As shown in Figure 2, the cassette should contain a reporter DNA...For this FLEx switch to successfully work, the cassette would need to contain the BFP coding sequence ...antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target sites-...in the opposing orientation, you have created a cassette that will express GeneR only in the presence of...recombination event would excise the reporter from the cassette, allowing for expression of GeneR. This allows...

  17. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...To overcome this obstacle, a positive selection cassette can be incorporated, and used during the differentiation..., but defective for integration [4], the donor cassette is removed leaving behind no accessory transposon...recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac™. Image courtesy of ...recombinase technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site..., it is possible to make the ITR and selection cassette disrupt the target site for the nuclease thereby...preventing re-cutting. Transposase removal of the cassette later restores the target sequence. This allows...

  18. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...resource for spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents...into conversations with other users. The entire dataset can be downloaded from Zenodo, including the reagent...table or by downloading directly from the Zenodo dataset.     Figure 2: Screenshot showing reagent...HuBMAP) antibody metadata standards. And the dataset is rich. Experimental data includes reagent type...protocols, panels, publications, software, and datasets. It’s also a great place for anyone who has used...

  19. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ... Biological General Repository for Interaction Datasets (BioGRID) in order to increase the accessibility...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and ...and ready access to stably archived large-scale datasets by computational biologists. Figure 1: ...other species. By accurately compiling genome-wide datasets, which are often obscured in large supplementary...but also allows cross-interrogation of different datasets that might otherwise be hard to identify. ...

  20. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...bacterial selection cassette for growth and cloning in bacteria and a mammalian selection cassette to screen for...cells, you will not need a mammalian selection cassette (e.g. 1435 pSG5L Flag HA). You can also upgrade...with an integrating vector expressing a selection cassette with 5′ and 3′ flanking sequences matching the...fusion proteins. Pick your promoter, selection cassette and visualization vehicle to conduct your custom...
Showing: 361 - 380 of 520 results