Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 612 results for: Sis

Showing: 361 - 380 of 612 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due...columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial...

  4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... HaloTag, TMP-tag) – These widely used systems consist of a genetically encoded enzyme variant tag that...fluorogens (Figure 1e). Stains – These labels consist of a fluorophores conjugated to molecular species...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...began engineering new versions of GFP through mutagenesis in order to improve its physical and biochemical...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...however, the protein integration host factor (IHF) assists with the process: it bends the DNA to redistribute...with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids may result in...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering to...  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield compact...

  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...screening, followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov...scripts provided by the depositing lab for data analysis.  I'm often asked whether this step can be skipped...

  11. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...alternative tRNAs Codon optimization With low cost DNA synthesis, one of the primary ways researchers solve the...created codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online...

  12. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...environmental pollutants, drug discovery, and disease diagnosis. As biosensors, aptamers hold a few important...largely in vitro; Easy and reproducible commercial synthesis (those of us who have used antibodies for diagnostics...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...

  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...the hippocampus. This technology has formed the basis of the Rett syndrome program being pursued at Vico...nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular...

  16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... well received, understand that people can be resistant to change. Ask your peers or your superiors to...to explain their rationale when they’re resistant to new ideas. If their answer is “we’ve always done ...

  18. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...image shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools were ...function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences ...

  20. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....
Showing: 361 - 380 of 612 results