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We narrowed to 688 results for: Tec

Showing: 361 - 380 of 688 results

  1. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...have a natural preference to stay in dark areas (protected) as opposed to brightly illuminated ones (risky...environments by initially moving along a wall (protected) rather than being out in the open (risky). Taking...comprised of a brightly lit (risky) and a dark (protected) area. When measuring the time spent in a particular...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol 33:139–...discovery through the creation of innovative technologies. To date his group has develop several popular...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...polymorphisms specific to their origins. This technique is still used today to test for HeLa contamination...test is so good that it is used as a forensic technique (aka DNA fingerprinting) that can place suspects...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...ability of reviewers (and, once published, anyone) to detect possible errors in assumptions, use of inappropriate...senior colleagues. Secondly, science is highly technical and this has a natural, albeit unintentional, ...Policy: Part Two U.S. Office of Science and Technology Policy (OSTP) memo Increasing Access to the ...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred method... their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org/10.1038/nbt... constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186/1472-6750-6-1...

  6. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...told all of the nuances of the research in as technical of language as possible. Seriously. My suggested...review of your field or the finer points of your techniques. You DO need to be prepared to know the literature...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three...witnessing and audit trails. How does the ELN provider protect and respect your IP rights and data ownership? ...interested in combining scientific research with tech advances. You an follow SciNote on Twitter @scinoteELN...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...with the contents of the two AAVs. Using this technique, Bok and Porteus deliver a repair template that...respectively. Future researchers could apply this technique to efficiently deliver large repair templates ...AAV vectorization of DSB-mediated gene editing technologies." Current gene therapy 16.3 (2016): 207-219....

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    .... Alternatively, you can perform simultaneous detection with a mixture of primary antibodies and appropriate..., M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex...Introduction to Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence...

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...repair of the break to allow for site-specific detection downstream. The con to these approaches is that...that NHEJ must perform the repair to allow for detection, not Homologous Recombination or Alternative End...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...

  12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ... Why might one be interested in state machine technology? Biological research has produced a massive amount...itself to cheap, quick, and high throughput state detection with flow cytometry. However, expressing reporters...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

  13. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...developing an easy to use diagnostic tool for the detection and diagnosis of different types of pancreatic...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

  14. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... good correlation between the levels of target detected in both methods. These approaches have relatively...are ultimately planning to use your antibody to detect endogenous levels of the target in other cells ...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002...

  15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    .... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

  16. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...even if they are present in the sample. Other techniques, like electron microscopy and FRET, can offer...details are still being uncovered, and different techniques all bring their own advantages—and blind spots—to...RNA-programmed Cas9 nuclease specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt...

  17. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ... looking for the best way to improve your lab techniques — but perhaps are a little limited on time — ...share quick tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new...

  19. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

  20. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ... at MIT. He also writes Codon, a blog about biotechnology.    ...
Showing: 361 - 380 of 688 results