We narrowed to 427 results for: Trac;

1. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ... is sedimentation equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers...

2. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   Published

   Feb. 25, 2016, 3:30 p.m.

   ...an excellent resource that has gained a lot of traction with researchers. It is an excellent way to meet...

3. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...

4. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...but they can also be expressed in bacteria and extracted with common protein purification methods. Read...

5. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...

6. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your...

7. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et...

8. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...generation of such resources in less genetically tractable organisms lags behind. BiFC can also be used to...

9. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...dire than Zimmer could have imagined. Does this detract from the quality of the book? It doesn’t need to...

10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...expression for the data likelihood contains an intractable integral of the product of a Poisson and multivariate...

11. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ...place.  When the PCR is complete, a droplet reader extracts the droplets from the plate and measures the fluorescence...

12. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    Published

    Sept. 6, 2022, 1:15 p.m.

    ...providing DOIs, this information can be cited, tracked, and appropriately credited, broadening the knowledge...

13. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...

15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...functions in the hope of developing drugs against intractable diseases. Currently, a number of labs are using...

16. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

17. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral...

18. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ...

19. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...This is normal and it means you’re on the right track!  Get feedback After you’re done editing, send it...

20. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance...