We narrowed to 635 results for: UTY

...yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine synthesis ...). PubMed PMID: 10436161. 7. Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010)....

...through qualitative data that they are a long-term solution to reproducibility and cost-effectiveness for ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

...versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells... like the addition of a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence...

...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...

...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

...enhanced response. Evolution of a new variant Maria and the team set up a directed evolution and screening ...

...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...

... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...

...explosion of software tools accompanying the CRISPR revolution. (See omictools for even more.) However, none...McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg...

...long-term imaging or advanced techniques like super-resolution microscopy. To make this segment of the color...on the application. Figure 1: Super-resolution imaging of actin filaments with mGold2. Top: ...

...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...

...decided this protocol would be a worthy first contribution to the protocols.io community. Did I mention...scientists workflow in mind and can offer a one-stop solution to your lab's organization and management needs...

...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...

...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine..... AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51...

... absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as ...elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9 fused to a biotinylation tag expressed on...

...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in...

...tools for human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons...