We narrowed to 635 results for: Uty

...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

...that you will need large volumes of lentivirus routinely, you may opt for simply increasing the number ...ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently, this may...

...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...provides on-site courses in public engagement for institutions and the Royal Society gives training in media...

...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

...versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells... like the addition of a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence...

...long-term imaging or advanced techniques like super-resolution microscopy. To make this segment of the color...on the application. Figure 1: Super-resolution imaging of actin filaments with mGold2. Top: ...

...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

...enhanced response. Evolution of a new variant Maria and the team set up a directed evolution and screening ...

... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...

...explosion of software tools accompanying the CRISPR revolution. (See omictools for even more.) However, none...McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg...

...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...

... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...

...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...

...decided this protocol would be a worthy first contribution to the protocols.io community. Did I mention...scientists workflow in mind and can offer a one-stop solution to your lab's organization and management needs...

...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...

... absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as ...elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9 fused to a biotinylation tag expressed on...

...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in...

...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine..... AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51...

...tools for human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons...