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We narrowed to 643 results for: Vars

Showing: 361 - 380 of 643 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...interest. Thanks to the tremendous efforts made by various research teams to build and constantly improve ...integrated, we look forward to the realization of a variety of perturbations and applications in functional...

  3. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

  4. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...overcome this, lentivirus can be concentrated by a variety of methods such as ultracentrifugation, centrifugation...Finally, choice of concentration method depends of a variety of factors such as resources available, cost and...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are available...gene of interest. Researchers have developed a variety of endogenous gene tagging systems. Addgene’s CRISPR-based...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies...

  9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing...out this post. Learn how to Choose the Best Cas9 Variant for Your Next Experiment Read more about Optogenetic...

  10. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...–10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1–10) Improved split fluorescent proteins...

  12. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...flexible nature of MoClo allows users to test a variety of different orientations and components within... allows researchers to assemble and test many variables all at once. CRISPR CRISPR arrays and libraries...

  13. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ... either 3-base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see... to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly, which used only ...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited...

  15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...process and includes Picogreen quantification of various intermediate steps, a sizing electrophoretic gel... the pool but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... these modules (linker length can and should be varied as it can have important implications for the fusion... Various Addgenies...

  17. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...researcher and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze...be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction efficiency...

  18. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  19. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ..., FLEx is currently being used to study a wide variety of in vivo conditional gene rescue experiments,...switches to conditionally test the function of various point mutations and truncations in the absence ...

  20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...share plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized...identify. There were dozens of different mushroom varieties alone. At least I could tell that they were mushrooms...
Showing: 361 - 380 of 643 results