We narrowed to 526 results for: arl

1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ... QC process. While this has worked well for the early sets of SARS-CoV-2 materials, it will not scale ...

2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...the ratio of pathogenic to non-pathogenic tau by nearly 50%, showing proof-of-concept for pre-clinical ...

3. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide...

4. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical feasibility...

5. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   Published

   March 23, 2017, 2:30 p.m.

   ...fulfilling our mission for an even bigger impact. In early 2016 we surveyed hundreds of scientists about various...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ...miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as APEX2. What’s more, since the enzymes...

7. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...teacher professional development program that trains nearly 100 teachers each year, with workshops held at ...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...light-based control of signaling pathways. This is particularly exciting because using light to control signaling...

9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...NG (Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to...

10. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...final check before publication.   Do research! Nearly everything we write entails a primary literature...

11. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...most abundant molecules in a cell, this system is clearly also robust enough to ensure high levels of gRNA...

13. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...test was the perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR...

15. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...challenge associated with molecular plant parts, particularly for applied projects intended for commercialization...

16. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast...

17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...knock-out mice, but for various reasons it is not nearly as efficient in human stem cells. What genome editing...

18. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...touched on fluorophores as antibody conjugates in an earlier post, where you can find some additional tips for...

19. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ...Building upon the PE2 and PE3 systems of their earlier work, they developed a system in which the cellular...

20. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...