...incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of doing the AMA were interesting...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science from LabLinks...

...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...numerous variants have been developed with different mechanisms to inactivate the integrase.  The IDLV life cycle...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...Kempski, H., Brugman, M. H., Pike-Overzet, K., Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, ...

...How are you currently exchanging information with your team? If you need to exchange information regarding...which they are sure that there will be no sharp changes in costs once they establish the system within ...consolidated to one place, easily retrievable, searchable and traceable. However, switching from paper ...

... of reasons to consider using genome editing to change the genetic code of plants, including the development... system in most organisms, some plant-specific changes to CRISPR components are necessary to enable genome...try your hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome.../mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016) The CRISPR/Cas...

... do you need [for your lab start up]?” A hiring chair or dean will often ask a faculty candidate. Sweaty...focusing on long term investments. For example, purchasing “used” equipment can save you money in the short...their tangible resources. For example, if a dean/chair asks “how much do you need to start your lab”. You..., working with this question is second nature. Channel your inner curiosity and use your research training...

...giving greater concentration of cut DNA without changing the size. As a result, a restriction digest of...reducing multimerization and define recA-independent mechanisms. Avoid overgrowing the bacterial culture Another...PMCID: PMC3060453. Bazaral M, Helinski DR. Characterization of multiple circular DNA forms of colicinogenic...Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. Epub 2009 Feb 20. PMID: 19233255...

... loci of integration [8].   Figure 1: Mechanism of the piggyBac™  transposase/transposon gene ...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

... called SNAPf. eDHFR: The Virginia Cornish and Michael Sheetz labs demonstrated in 2005 that proteins ...and see the results right away. Or perform pulse-chase experiments by sequentially adding different ligands...All this flexibility comes at a price, though. Purchasing lots and lots of organic dyes or specialized ...Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7...

... genome editing system you use, there remains a chance that the observed phenotype was caused by an off-target...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...: 237-243. PubMed PMID: 25664545. 10. Gabriel, Richard, et al. "An unbiased genome-wide analysis of zinc-finger...Therapy (2015). PubMed PMID: 26502778. 13. Frock, Richard L., et al. "Genome-wide detection of DNA double-stranded...

...in a screen, to ensure that each gRNA has a good chance to contribute to the screen. In the end, this results...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church (7). These reference libraries contain... 28162770. PubMed Central PMCID: PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering...

... research papers, blogs, free courses, YouTube channels, books, podcasts, and communities to help people...Niko McCarty; adapted from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly...understand the contours of each topic. The video channels are also underrated and I recommend checking them...

...description of the applicable research project when purchasing export-controlled materials. Addgene will then...our normal process.   Due to recent regulation changes, an export control license for rabies materials...processing fee will be removed before your account is charged.  Timelines  We have offered VSV and SARS-COV-1...

... the 2022 #DeckTheLab contest!  and it's..... Michael Früh submission, which we have entitled "Do You...friend anymore :C pic.twitter.com/rExV3b8Q8u— Michael Früh (@Michelderapfel) December 19, 2022   Congratulations...used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint. #deckthelab...

...Number of requests per reagents (as a horizontal bar chart) Number of requests per country (as dot plot) ...) Number of requests per year (as a bar chart) A written summary of the data, including total requests...microscopy (University of Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes...

...Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in the malaria community for the...it all settles down and the value of each item changes from what the reviewers so painstakingly assessed...space. The CRISPR research community hasn't had a chance to develop a consensus on the best CRISPR software... up with it. The peer review process has been challenged with many closely spaced CRISPR software submissions...

...these challenges, a group of stakeholders came together with the goal of driving systemic change in academic...the community.  Talk alone is not going to drive change. This effort is already resulting in concrete actions...

...created tagged versions of the cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions...with multiple other proteins. Again, little to no change in cell morphology was observed with IT5 and IT6...methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...

...Department of Energy’s Joint Genome Institute for uncharacterized genes that were proximal to a CRISPR array ...presence of a predicted RuvC nuclease domain, characteristic of CRISPR-Cas enzymes. In contrast to other...bacterial genomes but only in the genome of a group of Archaea. Consistent with this origin, the authors suggest...

...biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative to conduct subcellular...optogenetics. Even though GFP has been so thoroughly characterized, it turns out this protein has a few more secrets... LM, Wang Y, Condeelis JS, Verkhusha VV. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange...

... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested..., identifying these deletions, and finally characterization. Read and download the full protocol from ...Dass A, Yien YY, Chung J, Masuda T, et al. Characterization of genomic deletion efficiency mediated by...