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...The triple T’s of biology are transformations, transfections, and transductions! In this blog we will...they require delivery to be viral-mediated. Transformations are usually bacterial in nature. All three ...your choice. First, a viral vector is selected to perform the transduction with: either retrovirus, lentivirus...still express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection,...have protocols available to get you started and information on the specific viruses available to choose from...

...covalently joined, scarless DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications...designing primers with your desired mutation, performing PCR to generate the mutation and linearizing ...target region. Assembled plasmids can be directly transformed into E.coli for plating, screening and generation..., generating expression cassettes for yeast transformation can take 5 days. NEBuilder shortens that time...inserted into a linearized cassette, ready for transformation into yeast that same day.  The field of synthetic...

...mean read count per sample, and the amount of information per read does not vary by DNA input amount. plexWell...Sanger sequencing because the sample identity information is always displayed at the individual read level...targets. The NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous... with Addgene to develop a highly optimized bioinformatics pipeline that will return high-quality assembled...sequencing problems. He received his PhD in Bioinformatics from Boston University, and did postdoctoral...

...Vitae to showcase your publications, academic performances, and research experiences, you now have merely...different from a CV? Get familiar with the general format of a resume and the different presentation styles...summarizing what a resume should be about. With a CV, information is usually presented chronologically. With a ...almost missed because I had to “dig” hard for the information. Think "skills," not just "research and publications...Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that you have handled...

...will make it easier for you to share plasmid information with colleagues, or explain plasmid features ...sequences” button to find all available sequence information from Addgene depositors and our own sequencing...on the main plasmid page under the "Resource Information" section. Analyze your plasmid sequence For more...SnapGene and gain access to additional tooltip information and sequence analysis tabs including: Maps ...feature, enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ...

...couple of promising variants to check how they perform under your experimental conditions. This is time...of fluorescent proteins and how to make a well-informed decision on the best fluorescent protein for your...combinations of biology and equipment. Because the performance of a fluorescent protein strongly depends on ...by only a single amino acid. Without further information about properties like photostability or aggregation...endogenous tagged genes) can be used to verify performance under different imaging conditions (e.g. different...

...Neurons are really cool because they can transmit information, for example, from one of the 180 regions to ...(Fig. 1): one to visualize neurons that send information to a region (retrograde tracer, Fig. 1A), and...and another to visualize neurons that receive information from a region (anterograde tracer, Fig. 1B). ...into the brain and efficiently deliver genetic information or tools, making them useful tracers, but finding...exhibit retrograde transport and can therefore perform retrograde tracing, but often at the cost of toxicity...

...devices for research. There are a number of new platforms (such as openUC2, which I am a co-founder of),...that make everyday microscopy much easier. On platforms such as Github, users can download the software...example, can also be printed quickly in Madagascar. Platforms that allow for on-going creativity with the construction...organizations and companies to provide problem-solving platforms.     Fig. 3: Different workflows involved...Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and Deployment." bioRxiv. 2020. Diederich...

...Broaden your network Take advantage of formal programs A formal mentoring program can be a great way to... their information in these searchable databases are usually offering their contact information so you...know some of the people you work with Give more informed feedback (for example, they may be able to hear...

...their profile. Make your professional contact information available. When I am speaking at a new institution...requesting connections to complete strangers for informational interviews. Many have had success with this ...Send. That way you can type a full note with good format without accidentally sending before you’ve had ... This type of connection can help you gather information, make introductions, and give advice for your...volunteer organizational work. LinkedIn is not the platform for politics, family updates, or click bait of...

...submitting Freedom of Information Act (FOIA) requests for their postdoctoral salary information as of December...regions When we originally started gathering this information, many assumed that those living in areas with...the institution. Continuing efforts to gather information and expedite change We are continuing to update...update our FLSA and Postdocs resource as information becomes available, under “How institutional plans have...

...Academic Data and the Labor Market. For more information on the symposium as a whole and for registration...system operates and functions” “Complete lack of information on number of postdocs” “Postdocs are really hired...discuss where better data is needed to help shape and inform the overall structure of the biomedical research...clear that many important questions, areas for informed discussion, debate, and data collection still ...PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to reflect the workforce." Science ...

...do anything. Viruses are complicated and they perform complex biological tasks, both of which are factors...you’re unsure about the integrity of your virus, perform a diagnostic restriction enzyme digest and compare...transfected packaging cells. This check can be performed routinely and without disrupting the flow of your...viral vector. This antibiotic selection can be performed approximately 72 hours after transfection and ...Another method of ensuring viral production is to perform PCR against components of the viral vector using...

...previous formats but I learnt to overcome the personal frustration and submit reports in the format requested...and I made a point of sticking to deadlines and performing the work I agreed upon (just as I would in my...on behalf of the Advocacy Committee would change format depending on the particular style and requirements...Boston University School of Medicine and is the former Chair of the Advocacy Committee at the National...

...biophysics By Mike Lacy In order to study the conformational dynamics of the motor protein kinesin-1, the...Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo. bioRxiv 2024.12.20.629623... Figure 4: ONE-GO biosensors measure the formation of Gα-GTP by BRET. The biosensor consists of a...signaling in primary cells. The authors used this platform to assay dozens of GPCRs across many cell types...context-dependent GPCR activity with a universal biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org...

...Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded rates of targeted mutagenesis...introduction of DNA into therapeutically relevant, non-transformed mammalian cells. These viral vector systems also...This technology therefore represents a potential platform that could be realized via simple switching of...with your CRISPR/Cas9 research! To find more information about the adenoviral delivery of CRISPRS/Cas9...

... Jinek structure had caught the protein in a conformation that was not “poised for cleavage”. Jacob computationally...way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible plaster. As a result...of all proteins in general, and the amazing conformational flexibility that the Cas9 protein must have...suggests the potential of this protein to undergo conformational shifts. This backbone model of Cas9 is based... the middle of the night dreaming about the conformational gymnastics that this protein must undergo as...

...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...become a new way to allow you to get important information about the campus even if you can’t visit. By ... days during my midterm exams. Using the online format, I didn’t have to worry about traveling during ...interviews. I also had universities change their format of interview from a travel day to an online call...

...want to select one (or both) depending on the information you want to verify. We will delve into these ...sequences is probably the fastest BLAST search to perform and will save you time compared to other types ...name into the Organism box. This small piece of information can significantly reduce your wait time for blastn...parameter, as this will affect the amount of time to perform the search as well as the overall alignment results...sequences, particularly for comparing different isoforms. In addition to our favorites, there are a number...

...TnpB-mediated genome editing, and two of our top-performing constructs are now available through Addgene ...immediately downstream of the TAM. This RNA-DNA hybrid formation recruits TnpB to the site and activates its nuclease... your reverse complement guide (see Figure 1). Perform standard phosphorylation and annealing of the two... or M13R as the sequencing primer. Step 7: Transformation Your confirmed plasmid is now ready for Agrobacterium-mediated...Agrobacterium-mediated, biolistic, or PEG-mediated transformation into plant cells. Conclusion & Future Directions...