We narrowed to 611 results for: rev

...retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA...repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids...

...excluded from the system), these reactions are irreversible. Once expression of a particular recombinase... gene on a register. The purpose of this is to prevent interference between the transcribed gene and its...

... anneal to the backbone in either a forward or reverse orientation so you’ll need some way to verify that...digestion, you will need to use a phosphatase to prevent re-circularization of the backbone plasmid (see...

...really does not take much time at all. Much of the previous literature using AAVs for DREADD delivery indicates... of neurons showing a very specific phenotype. Previous neural tracing methods used viruses that were ...

... should review before using an antibody.  Antibodies and Reproducibility First, let’s review why validation...

...modification with an ssDNA substrate.  Gam: Gam prevents both the endogenous RecBCD and SbcCD nucleases...repressor gene, CI857, is used.  This mutant repressor prevents expression of the recombination genes at low temperatures...

...Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence Transitions.... Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics 48, 178-243 (2015). PubMed PMID: ...

...the original reference library, as well as GC%, reverse compliment sequences, and more. Output_Compact:...Wang, Tim, et al. "Gene essentiality profiling reveals gene networks and synthetic lethal interactions...

...excitation. After a short period, the electrons revert to a lower energy state, whereby a photon is emitted...markers they label) can occur. As I mentioned previously, you can also use fluorescent reporters, such...

...super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme...filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

...preparation, staining procedures, and controls. To prevent instrument clogs, ensure that samples are single... LA (1972) Fluorescence Activated Cell Sorting. Review of Scientific Instruments 43:404–409 . https://...

...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/... burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https://doi.org/10.1038...

... stem cells: reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. PubMed PMID:...reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation." Methods...

...primers: EMX1-Forward: CCATCCCCTTCTGTGAATGT EMX1-Reverse: GGAGATTGGAGACACGGAGA Q16: Why does the PX260 ...D., Lin, C., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang...

...expression of a catalytically dead Cas9 enzyme allows reversible repression based on the presence of the inducer...modulation. CRISPRa from sigma-54 promoters Noting that previous work in bacterial CRISPRa had focused on sigma...

...depositing their data with us through our newly revamped Data Hub! The community needs more data-sharing...

...Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing (...

...away from the functionality and features that previously made the platform a mainstay of conversation ...

...on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology, for example, the authors ...

..., the Phuels lab deposited a plasmid that will forever change the fly lab experience. The Frequency Light...