We narrowed to 430 results for: sequence

1. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   Published

   Sept. 3, 2020, 12:15 p.m.

   ... the dCas9-CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the...photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a double...

2. Deep Dive: qPCR

   Type

   Blog Post

   Published

   Sept. 29, 2022, 1:15 p.m.

   ...product on an agarose gel, then cutting out and sequencing the band for verification. Primer dimers will...uMelt is an online tool that uses the  amplicon sequence and thermodynamic and experimental parameters ...

3. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...transcriptional units. Individual parts are defined DNA sequences with specific functions. Most individual parts...

4. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ... except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA) are removed, resulting...

5. To Each HIS Own

   Type

   Blog Post

   Published

   March 2, 2023, 2:15 p.m.

   ... affinity chromatography against an amino acid sequence, or tag, on the protein. While a variety of methods...

6. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...namespace collisions you may encounter: vector, sequence, insert, trait, factor, expression, etc.There ...

7. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...is to knockdown a gene with RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA...

8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ... outcome are then photoconverted, sorted, and sequenced to identify the mutants for further enrichment...

9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...or chemogenetic receptor, through an IRES or 2A sequence. In this case, the proteins are not fused. The...

10. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016...

11. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...classifications are still being created as whole-genome sequencing becomes more relevant (Garcillán-Barcia et al...

12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ... incurable. Rapid advances in next generation sequencing technologies have allowed scientists to quickly...

13. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ...technique or experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand...

14. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...are often intramolecular probes, as the linker sequence between the donor and acceptor is sensitive to...

15. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked...proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic domains tethered to...

17. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...more than enough DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even...

18. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...tRNAs much thought. High throughput transcriptome sequencing methods including work done by the ENCODE project...

19. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ...variation. Confirmation of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal...

20. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...Knockdown library. Although B. subtilis has a fully sequenced genome, many applications in biotech, and is considered...