We narrowed to 432 results for: sequence

... the dCas9-CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the...photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a double...

...product on an agarose gel, then cutting out and sequencing the band for verification. Primer dimers will...uMelt is an online tool that uses the  amplicon sequence and thermodynamic and experimental parameters ...

...is to knockdown a gene with RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA...

...protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016...

... except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA) are removed, resulting...

... affinity chromatography against an amino acid sequence, or tag, on the protein. While a variety of methods...

...transcriptional units. Individual parts are defined DNA sequences with specific functions. Most individual parts...

...accessible simply by dialing up the right nucleic acid sequence (Addgene can help with this!). Besides requiring...

... outcome are then photoconverted, sorted, and sequenced to identify the mutants for further enrichment...

...or chemogenetic receptor, through an IRES or 2A sequence. In this case, the proteins are not fused. The...

...namespace collisions you may encounter: vector, sequence, insert, trait, factor, expression, etc.There ...

... incurable. Rapid advances in next generation sequencing technologies have allowed scientists to quickly...

...are often intramolecular probes, as the linker sequence between the donor and acceptor is sensitive to...

...classifications are still being created as whole-genome sequencing becomes more relevant (Garcillán-Barcia et al...

...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...

...interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked...proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic domains tethered to...

...technique or experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand...

...more than enough DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even...

...tRNAs much thought. High throughput transcriptome sequencing methods including work done by the ENCODE project...

...variation. Confirmation of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal...