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We narrowed to 904 results for: CAN

Showing: 11 - 20 of 904 results
  1. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...key transferable skill that can help you get more of what you want and can also strengthen your relationships...negotiating with my PI on how to spend my grant money (Can I attend this conference?) and with my lab mates ...going on vacation (New York City or Hawaii?). So how can you approach improving this transferable skill? Negotiation...approach to negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all...problem-solving exercise? Asking questions like the below can help you understand the perspective of all persons... person’s request? Your colleague is if someone can feed their cells this weekend - why? Do they have...Someone has to be the first author but another author can present the work at the next big conference - Is ...
  2. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ..., since they can differ. 48” x 36” tends to be the most common poster size, but this can vary as well....readers can navigate your poster and follow the logical progression of your work, and you can accomplish...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...university, you are likely aware of how different posters can be. You have also probably thought about why certain...catch the audience’s eye and draw people in. That can be accomplished with an exciting title and attractive...and potentially a photograph of yourself. A photo can be very useful in the event that you will not be ... your poster at all times, so other participants can identify you. You may also wish to make creative ...
  3. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...instance, how much force can be generated by conformational changes in proteins? How can information propagate... R bodies can repeatedly extend and contract in response to changing pH, and they can rupture E. coli...questions can be addressed using R bodies: simple, force-generating protein machines that can extend and...both wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...understanding these complex processes can’t be overstated, we can also learn a lot from Nature’s simpler...
  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...Papers can be saved to a Semantic Scholar library. You can add hashtags to library entries, which can help...You can also set up alerts for new publications from a particular author. Saving papers Papers can be ...1996, but is getting an update! The latest version can be found here. The new PubMed will become the default...paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following other scientists helps...they support. Monitoring these types of accounts can help you find papers related to a particular topic...papers, so naturally they tweet about papers too. You can follow journals related to your field of research...technically a way to save papers on Twitter, but you can bookmark a tweet in order to easily access it later...
  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... parts can be assembled into TUs is key to understanding MoClo. By a similar principle, TUs can be further...many parts can be assembled into many combinations. Even with current technologies, it can be extremely...pair with it can be queried by testing any number in the Level 2 assembly. These pathways can require a ... particular cloning technique. Though rarer, you can find MoClo that relies on Gibson and other non-Golden...cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical levels: individual...terminators, etc. Once selected, individual units can be combined into… Transcription units (TUs), which...composed of multiple parts transcribed together. They can express one or more gene(s), and typically begin ...
  6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...conference, the audience can help decide which questions get answered. “You can upvote a question and the... you can revisit the Q&A in the chat box. Presenting? Make it interactive! As a presenter, it can be difficult...session from conference to conference can be wildly different. There can be no presenter at their poster, ... “On the flip side, you can attend conferences for just a little bit. You can just go to sessions that... may have apps or interactive calendars that you can use to help plan your conference experience.One of...there was a Q&A section in the interface where you can type in questions for the speaker. “I think a lot...could share their notes together. Figure 1: Can you draw Spongebob from these instructions without...
  7. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...titer of a viral vector stock can vary based on transduction target and can also be altered by freeze-thaw... (which can include empty capsids that are devoid of a viral genome). Empty viral capsids can be formed...Different primers can have different annealing efficiencies (Wang et al., 2013). Although you can optimize your...exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more reliable... us information on three important factors. What can AAV titers tell you? Physical titer: the concentration...titer: the concentration of viral particles that can transduce cells. Infectious titers are typically ... after negative staining. Empty vector particles can be identified after negative staining and appear ...
  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...Antibiotic resistance can be scary to think about, but we can all do our part to learn all we can, and minimize...ways in which bacteria can become resistant to antibacterial drugs. Resistance can be intrinsic, meaning...infection before they can test for resistance. Growing and isolating the infecting bacteria can take 24 hours ... for millennia. What we can do, however, is fight smarter, not harder. We can utilize tactics like rotating... is important to note that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs and...to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing genes with...methods of ‘horizontal gene transfer’. Resistance can also arise from random mutations in the genetic sequence...
  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...standard curve can be used to determine the amount of starting material in your sample.  qPCR can also be used...concentration, unlike qPCR where results can vary across replicates. ddPCR can be used to quantify DNA sequences...an in vitro method in which a small amount of DNA can be copied many many times in a short time period.... and essential practice in molecular biology and can be used in a multitude of scientific techniques such...    Steps of PCR The beauty of PCR is that it can amplify DNA using only a short list of reagents and... polymerase is thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified...denaturing) and cooling (annealing) steps Taq polymerase can copy DNA between the primers using the dNTPs.  Let...
  10. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...optimization can be performed to both maximize the separation and reduce any background staining. This can include...clone names, which can allow you to search different providers for the same clone. This can be particularly... process, one must first confirm that a candidate antibody can detect the antigen of interest in the given...proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g. DepMap) – you’ll need...PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your antibody... this post, we will describe the approaches that can be used to determine selectivity of an antibody for...line approach Genetic knockout-based validation can conclusively prove the ability of an antibody to ...
Showing: 11 - 20 of 904 results