We narrowed to 798 results for: KIN

...fluorescent proteins to oligomerize is known as “stickiness.” Since the fluorescent tag should operate as... per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration...oligomerization in cells. In addition to measuring stickiness, several researchers have also collected data...characteristics are important, but have less priority than stickiness. In my experience, fluorescent proteins that ...independent of GDI-mediated extraction and actin-based trafficking." PLoS biology 13.4 (2015): e1002097. PubMed ... measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in single cells." PloS one 11.1 (2016): e0146789...

...really fit in anywhere and everyone who was doing the kind of work my lab wanted to do already seemed to know...graciously took me for coffee and spent an hour with me talking about the tools I could use, and some experimental...This was invaluable to me as a bench scientist working at an institution where training opportunities ...network at the meeting. We found our mentors by asking for help with the program on social media. Many...between mentors and Singletons can accelerate the networking timetable for the Singletons in their bins.  ...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...leaps. Binning Singletons is its own organization, working to make scientific meetings more productive for...

...differently if I would be speaking to a colleague in the office next door. So be kind, helpful, and thorough...

...University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate ... YFG. You could compare this mutant fly to one lacking the mutation in the same genetic background. In...express YFG in a new tissue type (more details on making a fly line in the next blog post). For example,...get the right cell type expression as opposed to making a new fly with hedgehog under a new promoter each...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...where I spent a couple of days talking to researchers about their work, picking up plasmid deposits, and trying...spend a large amount of my time meeting with and talking to graduate students, post-docs, and PIs. I won...enterprise work better and because I really like talking to people about and communicating science. The ...research at BU, I realized that I’m energized by talking to people about science and that I love explaining...running away screaming, but instead can involve walking alongside it and giving it a high five. While screaming...technical writing, teaching, presenting, analytical thinking, time management, information distillation, etc...transferable to many different fields. If you start thinking about preparing yourself for a career while still...

...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...intended mutation while taking advantage of the improved efficiency provided by blocking mutations. Essentially... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...

...experience working in a developing tropical country. This is why I set up a placement working in a rural...work-life balance compared to working in research. My previous experience working in Industry was really valuable...sustainable. After working at this company for several years, I was ready for a change.  Working abroad: Research...new science graduates and the pro’s and con’s of working in different sectors. Luckily, I have worked for... decide for themselves.  Here is my experience looking for the perfect job. Summer work experience in... explain why I changed jobs. I highly recommend taking on a similar position in your field of interest...and problem solving. I believe that is something lacking during degree courses; they never really prepare...

...insufficient blocking) or reduced signal (too much blocking.) If you’re having issues with your blocking, first...it, try making fresh running buffer. Old or improperly made running buffers can cause all kinds of odd ..., degraded or improperly mixed blocking buffer, or insufficient rocking during incubation steps.  Many...pattern in your background, try asking more experienced labmates or looking it up online. You’ll find many...ResearchGate’s western blot forum.  Blocking Don’t skimp on the blocking time! Fill the time with smaller...  Troubleshooting Blocking reduces the amount of background in your blot, so blocking issues can either...you can try either longer blocking times or higher concentrations of blocking buffer. If you see a low ...

...room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn.  Any time...how to get it, especially if introverted ), and networking (how to get people to remember you). What better...Watch other people give great talks We are not talking about lab meeting here. Lifeless presentation of... Series. Watch how these speakers talk without looking at their slides or notes. Listen to their stories...and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion...

...46 : How Kingdom ended up working in the life sciences and agriculture 5:47-9:35 : Kingdom's biotechnology...: Intro 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to...14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects...Seeding Labs is helping advance Kingdom's research 28:08-END : Kingdom's Thoughts on his time in Boston...family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best of the best...Biotechnology at Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata...research at Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University...

...presenting on building relationships (also known as “networking”), one of the most common questions I receive...starting to see the advantages of using social networking to start, build, and track professional relationships...professional contact information available. When I am speaking at a new institution, for example, I will often...d be much more likely to say  “Hey, thanks for looking me up on LinkedIn!” resulting in just a little ...inviting someone to connect, perhaps you should be asking yourself if it’s really a good idea.  Pro tip: ... unless you’re on a profile page. You might be looking at a list of suggested connections and you’ll see...complete strangers as it borders on my first rule of networking--”If it feels creepy, don’t do it.” It’s just...

...chat box and upvote If the thought of walking up to the mic and asking your question in a large conference...in case someone’s internet cut out. “ On networking Networking can be more difficult in the virtual format...organic networking,” she says.Depending on the meeting, there may be a “place” for networking within the...answered this way and the hurdle of getting up and asking in front of many people is lower,” says Bachle....,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question.”If the...in the chat box. That’s another benefit to note-taking: you can revisit the Q&A in the chat box. Presenting...the participants to learn about what they were looking to learn in the workshop so they could steer their...

...scientist who began working remotely recently? Most of us at Addgene began working from home last week... mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...week to flatten the curve, but working remotely can be more difficult if your work is based in the lab. ...sharing links to online yoga classes. Get outdoors. Taking a walk or a bike ride is a great activity for both...the desire and ability to, some communities are looking for bench scientists to assist in labs that are...volunteering to foster a pet, for example. If you are looking for some resources specific to self-care during...check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health. Work on personal...

... to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working with a tissue...analysis process. And ChIP itself begins at...  Crosslinking Once you have your sample, the first step is...sample processing and therefore will require crosslinking, which uses formaldehyde or UV light to create...complexes together until you want to break them. Crosslinking is necessary for most ChIP experiences, but ...determine concentration.  Protein Capture After crosslinking and sonication or enzymatic digestion, you’re... not be a good choice) and the amount of cross-linking can affect epitope availability.  Collect the DNA...then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with a PCR purification...

...extracellular environments, making it effective for analyzing real-time kinetics, such as binding, degradation... their bulk (~27 kDa) can perturb folding or trafficking and their detection can suffer from photobleaching...degradation, trafficking (Hall et al., 2012). Antibody-based tools can also be combined with the HiBiT tag...endogenous locus or used other expression methods. Cracking the code of 'undruggable' targets Researchers ...at Addgene! Following extracellular RNA editors Tracking factors that utilize exosomal transport is quite...oncogenic editing of its most common target, AZIN1, linking EV-mediated protein spread to enhanced malignant...highlights HiBiT tagging as a sensitive reporter for tracking proteomic cargo flow via EVs. Find ADAR-HiBiT ...

...time for me to start considering my future path. Talking to Addgenies has helped me get a much better idea... Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since he... really get me where I haven’t gotten myself.” Perkins noted that there is a more emphasis today about...those doors to be opened.” Addgenie Eric Perkins presented a poster a few years ago at a CRISPR ...away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she...assistant in a lab at the university that I went to. Making that switch from being a grad student to an employee...Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate school and...

...there's a big office vs. lab culture shock). I'm looking to see if there are any resources that can help...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene.... What causes the lab → office culture shock? Working in an academic research lab involves a few key ...your family. I talked to a graduate student at a networking event recently who referred to her lab as her...applicant asked if she would partake in “critical thinking” in the position to which she was applying. I ... stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch says, “[It] seems...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where... cell culture, I would often respond to friends asking me to dinner saying something like, “I’ll be there...ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning out... this post useful. After a bit of research and thinking about my own processes, I’ve broken down time ...until it was too late. I didn’t spend enough time thinking about the possibilities and how to prioritize ...the day, month, or even the year, you should be thinking about two main things: How useful the task is...time it will take you to complete the task When picking which tasks to actually do, you should err on the...

...afford them). I am talking about making sure the audience is interested, educated and talking as a result of...moderator should have a well-planned approach to making the event interesting and engaging. Long before...the pre-event interviews, the moderator should be taking good notes and discovering interesting stories,... panelists have the correct date, location and parking information and remind them about the event as ... a really lively discussion, I strongly suggest asking the panelists not to use any slides for the event...prevent any one panelist or audience member from taking over. My own personal rule is that I do not let...chose good panelists, they are going to be good at talking, so they will easily take 5 minutes to introduce...

...conference. Networking All of the things we've mentioned above could be considered networking, but here ...tips. Before the conference Networking is key even if you are not looking for a job - those connections...the elevator pitch, talks, the poster session, networking, and social media. The elevator pitch Before...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the ...bored, or particularly excited about? If you tried tweaking your pitch throughout the conference, what worked...the poster session, send them a follow up email thanking them for their conversation and connect with them...social media. Social media Before the conference Networking can be made easier through social media. Use ...

...human kinases remains challenging. Small molecule kinase inhibitors usually target soluble kinase domains...Human kinase domain constructs for automated bacterial expression The misregulation of human kinases has...recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed...Threonine kinases while YopH (residues 164-468, Plasmid 79749) enhances expression of Tyrosine kinases. All...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion would...Arumugaswami lab at Cedars-Sinai Medical Center has kindly constructed and deposited an unpublished set of...

...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...plasmid maps!" After thoughtful design, vetting, and tweaking, we’re excited to announce that our plasmid and...GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive...identities of most features labeled directly on them making them much easier to quickly identify. Improved ...With this more functional display, you can start thinking about your next cloning experiment early on. Beyond...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...similar hover over features. As with the map tab, clicking on two separate locations in the linear sequence...

... and then condensing that and making it accessible to students.” Making science approachable is often ...This involves going in front of a camera and kind of walking them through the steps of, for example, cutting...And I’m like, 'Well, I’m here so let’s do it!'" Working as a subject matter expert Caitlin started out ...job as an SME: “I write curriculum for students taking science courses online, which is a blast. I have...an introduction that gives a basic overview of kinematics in physics, to developing a lab about mitosis...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...courses encompass what they want to teach.” Besides working with students and faculty, Caitlin also collaborates...

...basic one, making sure that you have a good fundamental understanding of what you’re working on is important...years. We each have a unique “origin story,” but taking the first step into the world of academic research...glassware with brightly colored hazardous liquids. Working in a lab is nothing like a typical part-time or... and applied. The current pandemic serves as a striking example of the applicability and importance of...be unsure, it helps to have a vague sense of what kind of research you would be excited about. I know quite...do what they do. I would also highly recommend talking to other undergraduate researchers, as well as ... yourself into is really important.  As you’re talking to people, though, try to be conscious of who has...

...thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should be frozen in multiple... generally ensure cell line identity and purity making it easier for comparable studies to be performed...to check their identity. For example, perhaps a working cell bank has been made by a research institution... good recordkeeping, you need to have a way of tracking how many passages the cells have undergone and... any of the Master cell vials until all of the working vials have been used up. This ensures you can retain...means you can regularly discard the cells you are working with because you have plenty of maintained freezer...freezer stocks. It is vital that the Master and Working stocks of cell lines have the passage number recorded...

...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the...tools allow one to study the kinetics of cell migration and movement by marking cells at a specific time.... Pathogens - Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization...tissues, including lymph nodes, brain, liver, gut and skin. Two-photon intravital microscopy. The video above...that some are more reliable and more efficient at tracking cells. Good two-photon probes when using a standard... cells and follicular dendritic cells within the skin and lymph node respectively. The permanent labelling...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     The past...

...release IL-36 cytokines. Keratinocytes are the main source of IL-36 cytokines in the skin making it important...the uncontrolled activation of the interleukin-36 (IL-36) cytokine family resulting in the activation ...disease research. This year is our fourth year working with the Rare Genomics Institute by providing plasmid-based...pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000...surrounding IL-36 biology. “Little is known how IL-36 cytokines actually get out of keratinocytes and induce their...researcher at University Hospital Zurich. IL-36 cytokines are a potent inducer of an autoinflammatory loop... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

...qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind of plate they...professionals from other backgrounds at conferences or networking events. And if you’re a wet lab scientist who...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...they were talking about during the meetings. And “plate” certainly wasn’t the only problematic word. How...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...creating shared experimental protocols. Clarify: Asking for clarification during a meeting can help get...

... worth taking time to honestly evaluate your own style of work and communication before making this important...attention you need? Are you good at asking for help? If you are good at asking for and getting help, size might... toss data-driven reasoning out the window when making decisions. We think scientists make choices based...based on emotions and assumptions. I’m not just talking about simple things like “Which route should I ...members can give you a fuller picture. Consider talking to people in the lab across the hall or in the ...prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...t choose a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting...

...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...type of team can exist indefinitely. If you’re looking for this type of team in academia, look no further...an imaging core or tissue culture core, you are looking at a process-oriented team serving a particular...you find that there is not someone (or someones) taking on these responsibilities on a team to which you...is certainly necessary, but equally important is making sure all team members understand what roles they... none of that matters. If you are on a team, any kind of team, and you have nagging doubts that someone...