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Showing: 31 - 60 of 269 results
  1. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...attention you need? Are you good at asking for help? If you are good at asking for and getting help, size might...you want to know and display character traits and skills that you hope to grow.  Find mentors who take their...diversity in all forms? How are their organizational skills? Are they involved with colleagues and the department...    Will you be encouraged to develop transferable skills by attending career events or being involved in...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...have access to information from other labs with skills that you can learn? Do lab members work on more...on the Addgene blog Check out our transferable skills guide Learn about careers in science communication...
  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...cell and 2. prevent reinfection of those cells. Kaminski et al. designed gRNAs to target the HIV-1 5’ and...Importantly, no off-target effects were detected. Kaminski et al. observed no negative effects on cell viability...up to 7 mismatches to the gRNA target sequence. Kaminski et al. next tested their procedure in HIV-1 infected...the odds of developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed...References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K,...plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang.... PubMed Central PMCID: PMC4649911. Khalili K, Kaminski R, Gordon J, Cosentino L, Hu W. Genome editing...
  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...at the University of Alberta studying with the Szymanski lab. Reasons to study how a phage recognizes its...useful and cost-effective method that our lab (the Szymanski lab, which has recently moved from the University...a technique published by Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to...protein! First, block your membranes with BSA or skim milk as you would for a Western blot in order to...at the University of Alberta studying with the Szymanski lab (though she’s tagged along with the lab during...David J., Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded...David J., Jessica C. Sacher, and Christine M. Szymanski. "Development of an Assay for the Identification...
  4. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...are rarely chosen because they have demonstrated skill at managing and this is especially true in science...good manager. The kind of smarts and the type of skills that it takes to be a good scientist are not the...on Emotional Intelligence or to hone delegation skills, for example. So aside from a focus on removing...an open mind to be willing to learn and grow in skills. Managing a team is hard and most people have some...do before they can be good at it. Like any other skill, it can be developed with education and practice...introductory reference books on managing people: Skills for New Managers by Morey Stettner Successful Manager's...
  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...While a PhD equips you with exceptional technical skills, transitioning into management often means stepping...problems in the lab, management involves a different skill set: mentoring, setting strategic goals, and fostering...cohesive team environment. Neglecting communication skillsClear communication is the backbone of effective management...team member who had values, working styles, and skills that were quite different from mine. Learning to...a supportive environment to build my management skills. Finally, developing emotional intelligence is ...What Makes a Good Leader Anyways?  Transferable Skills Guide: Managing a Team  ...
  6. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...all build and develop skill in science communication over time.  Like most skills, great science communication...that communication is the single most important skill that scientists need to succeed in their work. While...theory. So, what can you do to ensure that your skills in science communication will be a positive asset...practice. You can bootstrap your science communication skills with training programs, which are increasingly ...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...
  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed that ...that are found in and on everything, including your skin. Here are some general tips for working with RNA...this no-kit method are still widely used today (Chomcynski and Sacchi, 2006).  View our protocol for RNA...5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA ...https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of ...
  8. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...to develop the manual skills at the regular microscope, and the scripting skills at the automated microscope...and our research goals in focus. Developing these skills relies upon biologists and computer scientists ...but don’t really know how to start applying their skills to it. The Kittybiome project gives them access...traditional biology lab, we have members with a wealth of skills to help do science on the cheap using equipment...obsolete amazingly fast, but with a wide range of skills among our members we’ve managed to learn a lot ...
  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral & Helinski, 1968), in which the plasmid was ..., taking many forms (Levene, 2009; Higgins & Vologodskii, 2016). Plasmid multimerization occurs via homologous... PMID: 21285356; PMCID: PMC3060453. Bazaral M, Helinski DR. Characterization of multiple circular DNA ...doi: 10.1038/294184a0. PMID: 7029308. Goebel W, Helinski DR. Generation of higher multiple circular DNA...PMID: 4884687; PMCID: PMC225270. Higgins NP, Vologodskii AV. Topological Behavior of Plasmid DNA. Microbiol...
  10. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...other advantages to practicing effective delegation skills: The team will be stronger and less dependent... Are you delegating to someone with sufficient skills and experience? Do you think you are giving this...allows you to make the best use of your time and skills. It also ensures that other people in the team ...Delegation: Developing This Essential Managerial Skill" Additional Resources on the Addgene Blog Read ...Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual...
  11. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...channel just for asking people if they can write or review things and another for asking specific science...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and...information on scientific literature, technical skills, and experiences from thousands of laboratories...
  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...  Show demonstrated skill of people and/or project management  Bolster your skills in strong relationship-building...career, I’d have realized it was a great fit for my skillset and interests. As such, I want to be able to share...interacting with others, and have strong communication skills to tailor your message to any type of audience....companies, try to find internships with translatable skills: working with data, working with people, working...
  13. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...career into a series of stages to refine and build skills overtime for success. Many people will have different...not the only place to develop “industry related skills”. Many career paths in academia as well as the ...should look broadly at opportunities to develop skills related to their areas of interest. Additionally...first person to identify a novel protein found in skin cancer and your continued work is necessary to create...replacement).  In addition to presenting your expert skills your letter should include technical information...Additional Resources on the Addgene Blog Transferable Skills Guide 6 Steps to Submitting a Resume That Gets ...
  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... and the way it is funded and to learn tangible skills necessary for affecting change. On Friday, the ...reception. Saturday’s workshop will be an advocacy skills “bootcamp” for a focused group of participants....require an engaged sector with effective advocacy skills. Advocating for Science Symposium and Workshop ...participants develop the efficacy of their advocacy skills. Friday’s symposium will include a discussion on.... University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications Specialist...
  15. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...9514755. Richard Gronostajski: NFI-related diseases of neural development Richard Gronostajski is a professor...Paula Videira, Michael McMurray, and Richard Gronostajski who each are being awarded free plasmids form...they affect brain and muscle development. The Gronostajski lab has generated mice wherein it's possible...Campus in Aurora, Colorado, USA.     Richard Gronostajski is a professor at the University of Buffalo....
  16. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... profession: 1. Build on your existing skills & passion  Skills such as research, collaboration across...development experience and to build upon your existing skills.  2. Find your niche Identify and develop an area...Development Strategy to increase your competencies and skills in the grants profession. Your professional development... to other grant professionals and to build your skills and competencies. Becoming a grant professional...
  17. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...future. 3. Identify the skills needed to use these tools Outline the knowledge and skill sets needed to do ...Ascribe a cost structure associated to these tools, skills, and personnel Define pricing according to all ... and limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal...manageable laboratory environments. His business skills enhance his passion for science. Follow him on ...
  18. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... as this is increasingly reliant on programming skills which are not my strong point. Working in industry...GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however, some...there can be a glut of individuals with similar skill sets. This is not to say that all commercial companies...aspects of the company as well and use my other skills and interests. I was so glad when my future supervisors...does bring its own challenges. I’ve had to become skilled with technology like Skype in order to have meetings...
  19. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ...management needs. How Crystal developed her management skills - training, learning from peers, and being receptive...importance of recognizing and leveraging your unique skills set while also adapting to needs as they arise....How Crystal developed her science communication skills and how they've helped her at Novartis. The importance...importance of mentorship in developing career skills. Outro Many thanks to our guest, Crystal Shih. Crystal...
  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...and when to terminate a project are fundamental skills that all group leaders must master. While the calculus...mentors) can never be too well practiced or too skilled at presenting and answering questions. Organizing...presentations, you will become a more confident and skilled speaker. There's a more fundamental reason to give...that is orthogonal to their scientific research skill set. You'll need to motivate your trainees, communicate...Non-Academic Career Decisions Check out our transferable skills guide Resources on Addgene.org Learn more about...
  21. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...you could be honing your science communications skills and networking. And on social media, you could ...have to create opportunities. If you find yourself asking, "Why doesn't this product/organization/position...Read all careers blog posts Read our transferable skills guide Resources on Addgene.org Find job listings...
  22. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...boards and check them regularly Use your research skills to determine which job boards list positions interesting...clearly generic cover letters that expound on bench skills and experience. Most Addgene PhD level job descriptions...should also be customized to highlight different skills and to get the important information in the right...strengths in your resume and don't be shy about asking for help. The worst thing that can happen is someone...
  23. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ... to obtain a “crash-course in all the real life skills that employers love PhD students to have: working... a PhD teaches analytical and critical thinking skills that are applicable to a variety of careers outside... who remain connected to scientists and use the skills they learned during their studies. They suggest... extracurricular activities both to develop new skills and to discover what you are truly passionate about...
  24. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... a teacher was looking for an outlet. I started asking people: why do you do what you do? How do you like...forming a different constellation of interests and skills than what fits for applied research. I focus in...consider an internship or a short program to build skills and connections. AAAS offers a summer program that...are a lot more planets to choose from. Put your skills and interests into your backpack, and step out ...
  25. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork       ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...request for visitation if they have specialized skills and equipment! Preparing for your visit   Design...
  26. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...upvote If the thought of walking up to the mic and asking your question in a large conference room puts butterflies...answered this way and the hurdle of getting up and asking in front of many people is lower,” says Bachle....,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question.”If the...example, by using short animations and visuals, or asking questions using the polling feature or the chat...
  27. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...towards my PhD project. Grant writing offers various skill building opportunities that graduate students and... Improves technical writing and communication skills, Elucidates the big picture and future directions...continuing to apply for funding improves the associated skill sets. Increased experience in communicating and ...process and continue to improve your grant writing skills. Good luck! Further advice on grant writing Since...
  28. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...doctoral dissertations. Streamline the question asking process Having thought through the above, don’t...soon as possible!” and I always begin my responses asking for more specifics. We can help you faster if we... you email us at noon and then call back at 1PM asking why you haven’t received a response, just keep ...how long it will take to get an answer, consider asking to avoid unrealistic expectations. And finally...
  29. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...your 1-hour presentation to your audience Start by asking the hiring manager or recruiter what presentation...accomplishments and demonstrate your science communication skills. Know your audience. Connect your prior work or...opportunity to demonstrate technical and transferable skills that make you qualified for the position. Highlight...sharing your screen and won’t be able to see who’s asking a question. Maybe you ask for questions after completing...
  30. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...pool. FP timers have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This... course of 18 hours in vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics...cumbersome. 3. Portability across multiple systems Terskikh et al. showed that their versatile timer could...in the comments section below! References 1. Terskikh, Alexey, et al. ""Fluorescent timer": protein ...
Showing: 31 - 60 of 269 results