We narrowed to 65 results for: IRES-GFP

... enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat...of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline induced expression...

...gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications requires multiple recombination...to transformation (Figure 1). As this approach requires no special reagents or purification kits, it is...

... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage... tracrRNA and crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage... PFS. This is in stark contrast to Cas9, which requires that each DNA target have high sequence identity...

...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...

...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...

...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...another factor to consider. If your application requires longer periods of exposure, you’ll want a more...

...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...promoters for transcriptional regulation, so it requires a different approach for regulating transgene ...Generating iPSCs with plasmid-based expression requires serial transfection of 1 or 2 plasmids that express... the gold standard instead. Teratoma formation requires injecting iPSCs into immunodeficient mice and ...suited for these situations. The second scenario requires higher levels of safety and as little chance of...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...

...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

...original CAPTURE method The original CAPTURE method requires the creation of a stable cell line that co-expresses...expression. Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these...lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ...

...and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....