We narrowed to 174 results for: ADAP
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TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....Choose from different “Options”, including adding adapter sequences for cloning purposes. Press run and... well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all the information...subsequently cloned into your vector of choice using the adapter sequences that you have specified. Run your...
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Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....Table 1: Features of various qPCR chemistries. Adapted from Wong, 2018 Primers Design of primers...method is the Standard Curve method. See Table 2, (adapted from Wong, 2018) for a comparison of different ...comparison of different qPCR analysis methods. Table adapted from Wong, 2018. 🔥Hot Tip🔥 Negative... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....Cas14 targets and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene...discovered in 2017. Now Dominik Niopek’s lab has adapted anti-CRISPR proteins for optogenetic control of... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....Last updated Apr 17, 2020. Before being adapted by scientists to edit the genome of virtually any organisms...on this planet, CRISPR-Cas systems were merely adaptive immune systems that provide bacteria protection... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m...., the ability to embrace serendipity fosters adaptability, a crucial skill for a successful research career...research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....antibody-dependent cellular phagocytosis (ADCP). Figure adapted from van Erp, et al., 2019. Antibody-dependent...Song, WC. Complement and its role in innate and adaptive immune responses. Cell Res 20, 34–50 (2010). https... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023). To develop... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m.... effect is not specific to sucrose, but can be adapted to other sugars such as sorbitol. As sorbitol is...starters, we believe that this approach can be adapted by vector cores and laboratories alike as an easy... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....RNA-seq was performed to map TFs to cell fates. (Adapted from Joung et al.) This atlas linked TF ... color coded nominated reference cell types. (Adapted from Joung et al.) Putting MORF to work ... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022. Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....Nature Communications 2019. Pubmed PMID: 31366908 Adapting sigma54-dependent transcription for CRISPR activation...must be σ54-dependent and the system may not be adaptable to distantly related species from E. coli. This... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....high school classrooms, but it could be widely adapted and used in a variety of settings. If you are a..., if you are a fellow researcher interested in adapting your research ideas into hands-on educational ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....their devices, which makes it very difficult to adapt the device to one's own samples and limits the use...to as 'frugal science', existing components are adapted and combined with creativity and feedback from ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering endeavors. ... Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas proteins... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to instead drive...delivering gene editing tools into cells. Image adapted from Segel et al. 2021. Prime editing improvements... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....for assembling the barcoded gRNA library pool. (Adapted from Wong et al., 2016). With a list of gRNA...assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016). With CombiGEM and ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics...