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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 200 results for: ADAP

Showing: 1 - 20 of 200 results

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...exposure to a pathogen, the adaptive immune response kicks in. The adaptive immune response is antigen-...Tpl-2, c-COT NCK1 NCK adaptor protein 1 MGC12668, NCK, NCKalpha NCK2 NCK adaptor protein 2 GRB4, NCKbeta...contains plasmids for genes involved in innate and adaptive immune signaling and cell-cell communication. ...two systems that work in concert: the innate and adaptive immune systems. Upon infection, the immune system...cells also process and present foreign antigens to adaptive immune cells, such as T cells and B cells. About...includes cellular and humoral immunity. Once an adaptive immune cell is primed to recognize a specific ...of pathogens. Over the course of an infection, adaptive immune cells will expand and give rise to memory...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...hypothesized that prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated...Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive immune system seems like a winning strategy to...basic research. The beauty of CRISPR is that it’s adaptable to almost any model system or biological topic...programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science . 337(6096):816-21...

  4. Arf GTPase Family

    Type
    Collection
    ...(HRBL, RABR) 3268 481 GAP Adap1 (CENTA1) 11033 374 Bacterial (pET) GAP Adap2 (CENTA2) 55803 387 GAP Smap1...

  5. CRISPR Guide

    Type
    Collection
    ...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign...target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most popular genome...recruit activation proteins. CRISPRi has also been adapted for diverse bacterial species using a modular system...programmable Dual-RNA–Guided DNA endonuclease in adaptive bacterial immunity. Science , 337 (6096), 816–...

  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins expressed from their...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...CLOVER Center (Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...stimuli like hormones or metal ions, scientists have adapted an antibiotic resistance operon from E. coli : ...

  13. Plan Your Experiment

    Type
    Collection
    ...different Cas proteins available, and researchers can adapt these experiments for different organisms. This ...

  14. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant adeno-...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-... the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature...proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang...

  15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...more quickly and efficiently, they’ve recently adapted the CRISPR/Cas9 system for protein tagging. We .../crispr/tagging. Addgene: What motivated you to adapt CRISPR for use in protein tagging? Myers: Our labs...transcription factors. Our groups got together to adapt CRISPR for tagging experiments and it’s already ... improvements in efficiency and throughput. In adapting the CRISPR/Cas system, what we are doing is adding... is so flexible. Other investigators can easily adapt these vectors for use with their favorite selection...who’ve developed their own system or who use and adapt our reagents to think about adding them to Addgene...

  16. Typing CRISPR Systems

    Type
    Blog Post
    ... all CRISPR types; the fast evolution of these adaptive bacterial and archaeal immune systems has led ...suspected that they don’t carry out the same types of adaptive immune functions that other CRISPR types do, due...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ...pyogenes) belonging in II-A. Cas9 was the first to be adapted for use in mammalian systems, with the landmark... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...

  17. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857. Image adapted from Aggarwal et al., Figure 1   Increased...curves for the different iGluSnFR variants. Image adapted from Aggarwal et al…, Supplementary Figure 3. .../F0 for the different iGluSnFR variants. Image adapted from Aggarwal et al…, Supplementary Figure 2. ... PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… Figure 4. PDGFR is a C-terminal...

  18. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv, under CC-BY-NC-ND... B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY 4.0 license...(C) is indicated; red line is exponential fit. Adapted with permission from Hao and Lee et al., 2024. ...non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 license...

  19. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...purification of a specific genomic region, numerous adaptations of this system have been made in different fields...specific genomic region has also recently been adapted to isolate genomic loci in bacteria (Fujita et ...also has great potential for improvements and adaptations that can provide an increased understanding of...programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." Science337.6096 (2012): 816...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...Schematic of Opto-katanin’s use in cells. Images adapted from Meiring et al., 2022, under CC-BY 4.0 license...blue light illumination. Scale bar 25 µm. Images adapted from Mahlandt et al., 2023, under CC-BY 4.0 license...vesicle trafficking via CRY2-CIB1 clustering. Image adapted from Aljiboury et al., 2023, under CC-BY 4.0 license...colocalizing with SuperPLDx30. Scale bar 10 µm. Images adapted from Tei et al. 2022, bioRxiv, under a CC-BY-NC-ND...
Showing: 1 - 20 of 200 results