We narrowed to 79 results for: CHL

1. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ...

2. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...

3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45... not what most readers want to read. As Susanna Bachle points out in her talk (29:31 - 49:45), there are...

4. Live and Let Dye: Self-Labeling Protein Tags

   Type

   Blog Post

   Published

   April 16, 2024, 1:15 p.m.

   ...haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current standard version known...  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine...

5. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   Published

   Oct. 17, 2019, 12:28 p.m.

   ...for phototaxis in the unicellular eukaryote Chlamydomonas." Nature 311.5988 (1984): 756. PubMed PMID: ...Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991): 489. Hirsch, Matthew...

6. A Primer on Optogenetics: Introduction and Opsin Delivery

   Type

   Blog Post

   Published

   Sept. 10, 2015, 2:30 p.m.

   ...yellow-light sensitive chloride ion pumps, activation of which results in efflux of chloride ions, subsequent...

7. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

   Type

   Blog Post

   Published

   Nov. 3, 2015, 3:30 p.m.

   ...or commercial workbenches such as MacVector or Benchling. Utility functions: These functions help speed... so-called genome editing workbenches such as Benchling (commercial, online), DESKGEN (commercial, online...

8. Plasmid Preps: Different Purity, Different Quantities, Different Uses

   Type

   Blog Post

   Published

   June 16, 2020, 1:25 p.m.

   ...agents as guanidine thiocyanate and guanidine hydrochloride used during plasmid extraction. Figure...

9. Microbes: Look, Listen, and Tell

   Type

   Blog Post

   Published

   Aug. 28, 2018, 12:04 p.m.

   ... Susanna Bachle...

10. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type

    Blog Post

    Published

    June 23, 2020, 1:15 p.m.

    ...By Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share...

11. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type

    Blog Post

    Published

    Nov. 27, 2019, 2:03 p.m.

    ... Susanna Bachle...

12. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type

    Blog Post

    Published

    Sept. 15, 2014, 6:05 p.m.

    ...but when I met the astronaut Jim Lovell I was speechless (the club of people who have been in space is...

13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    Published

    June 11, 2020, 1:34 p.m.

    ...lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements...

14. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type

    Blog Post

    Published

    July 18, 2017, 12:41 p.m.

    ... Susanna Bachle...

15. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl...

16. Plasmids 101: Origin of Replication

    Type

    Blog Post

    Published

    Nov. 11, 2020, 2:23 p.m.

    ...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working...

17. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    Published

    Aug. 11, 2020, 1:15 p.m.

    ...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

18. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    Published

    June 2, 2020, 1:15 p.m.

    ...sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled...

19. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    Published

    June 27, 2018, 1:16 p.m.

    ...genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr...

20. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...References and Resources References Romano E, Baumschlager A, Akmeriç EB, Palanisamy N, Houmani M, Schmidt...