...collection of plasmids for coronavirus research, including a handful of plasmids that have been in the repository... plasmids used for this study at Addgene which include a C-terminal deletion in the spike protein to improve...Plasmids deposited from Alejandro Balazs’s lab includes those for generating pseudovirus and those encoding...the widely used qPCR and antigen tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had...for detection have been made openly available including those from Andrea Pauli and Julius Brennecke. ...19 diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase ...untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection...

...results, conclusions, and acknowledgements sections. Some other additions you might wish to include are a...navigable and efficient; your questions, data, and conclusions should be clearly identified and interpreted ...smaller than in other poster sections, so you can include a lot more. Font size: Making your poster readable...equally-sized columns, where the introduction, methods, conclusions, and acknowledgements sections are inserted. ...the central region, and a scannable barcode is included that links readers to more information if they...background but want to add a bit more “flash,” you can include a subtle gradient (as is shown in all of our illustrations...

...intentionally excluded any libraries from the software, and my hope is to continue including other libraries...strategies beyond SpCas9, or species not currently included in Green Listed.  Provide the list of genes ...Input” box. Choose from different “Options”, including adding adapter sequences for cloning purposes.... output folder containing several files. These include: Output: A text file containing full information...information about the suggested gRNAs to use, including all information provided in the original reference library...Output_Compact: A text file that describes which genes were included, and how many gRNAs were identified for each gene... gRNA, as well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all...

... open access publisher has required authors to include a note as to where readers may locate data supporting...information) for assessment of veracity of the conclusions drawn. The main difference with the PLOS policy...the time of manuscript submission. The rationale includes increasing the ability of reviewers (and, once...information is made available to everyone at once, including the generators. This practice doesn’t appear to...scientists are, however, privileged in having exclusive access to data and enjoy reasonable time to make...The fact that so few authors now query what to include suggests pragmatic adoption (although it might ...access to data is important, what data should be included? We should recognize that storing data in a sharable...

...data, you can even include your own data in a ReFigure. For example, you might include a confirmatory image...cofounder of ReFigure. Reading and exploration including replications and experiments resulting in “negative...also eLife, PLOS and Figshare). Some use cases include: Cataloging a reagent’s performance as in this...remain hidden from research papers as they are not included in traditional publications. By submitting your... currently supported by ReFigure beta) you can include it in a ReFigure. ReFigures are good for you and...

...sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats...chromosome encodes a tracrRNA (in some systems including Cas9), Cas proteins, and a CRISPR array. The CRISPR...is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria, these ...DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “single-...RNAs” are transcribed from the CRISPR array and include a spacer and a repeat sequence. The spacer sequence...mismatches can be tolerated outside this region. Conclusion Ready for some more details? Check out the Addgene...

... allows it to be used in more E. coli strains including the popular BL21(DE3) (Li et al., 2021). This ...original pCas/pTargetF but has many other advantages including shorter plasmid curing time. pCRISPomyces for ...Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces.... INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the...essential genes in a wide variety of bacteria including in pooled, genome-wide, formats. Interested in...proteins involved in the activation of transcription including transcription factors, phage proteins, and RNAP...Adding complexity, they found that they could also include a CRISPRi system for multiplexed modulation. CRISPRa...

...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies and...antigen, and performance in various applications are included in the full webpage. Educational Resources...discussed on Addgene’s blog and Antibody Guide, which include information about immunofluorescence, conjugates...over time. Through our incredible community, including your support, we have enabled scientists to easily...

...many roles that actively require these skills, including: Science Reporter Scientific Sales Epidemiologist...Careers where you’ll actively use these skills include: Science Reporter Scientific Illustrator Research...outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant...etc) are highly desirable in a range of roles, including: Science Reporter Technical Writer Statistician...position. Questions you’ll want to find answers to include: Will you be sitting at a desk all day or be on...to the content in your Resume and Cover Letter. Include any relevant job specific ‘buzz words’ to ensure...

...from the company LinkedIn page. This can include news, inclusion, culture and connections. Start your interview... is within a few days after you’ve met. Always include a note about where you met IRL (in real life) and... a connection request to any of these people, including and especially the hiring manager. This is considered.... Follow all the rules of good resume writing. Include examples of concrete accomplishments in both the...the technical and “plus skills” areas. Include a professional picture. It doesn’t have to be a headshot...

...results that ultimately builds up to an overall conclusion(s). In some articles, the data are presented ...to build a case for a certain model or overall conclusion. In others, the experiments are geared towards...possibilities until the results focus in on a particular conclusion. Many papers aren’t always written this way and...the results to see if it leads you to the same conclusion. Personally, I like papers that can end with ...are appreciated) to visually sum up all of the conclusions. In any case, the reader should give a critical... the relation to how they are supporting the conclusions. Look at the figures and write down your own ...

...genes that regulate a wide variety of phenotypes, including but not limited to cell growth and drug resistance...activate Ras are found in one third of all cancers, including a high percentage (up to 95%) of pancreatic cancers...crowd-sourced map of the RAS signaling pathway and includes 180 unique transcripts. Each gene was chosen to...provided in both open (no stop codon) and closed (including a stop codon) formats. The collection is compatible...collection of over 30 promoters and 140 genes, including constructs with unique tissue-specific promoters... a new method for generating protein chains or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016...key host for the production of their products, including fermented beer and wine, fuels, pharmaceutical...

...experimental processes, results, and subsequent conclusions. Being vigilant about these errors is an important...easy to share. However, the cloning process — including the propagation of plasmids in bacteria — has ...and money on optimizing experiments, to false conclusions.  Figure 1. Potential points where DNA..., indicated by exclamation marks. Error points include places where DNA is being created, such as PCR ...believe may affect the function of the plasmid. This includes point mutations, frame shifts, missing elements...require additional attention during analysis. These include topics such as: locations in a plasmid that are...

...genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic or .... We do this through a variety of activities, including creating educational materials and lesson plans...to hamper efforts to engage these communities, including those by researchers hoping to increase community...leadership and communication experience. These included taking extra coursework and attending seminars...of organizations in public engagement efforts, including giving public lectures, writing science articles...our experiences in a variety of other settings, including “conventional” academic venues such as scientific...

...of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary at the end if you...reaction performed by CBEs. However, most CBEs include an inhibitor of base excision repair (BER), the...Multiple labs tried to identify beneficial factors to include in even better cytosine transversion editors. New...proteins had additive effects when multiple were included in the CGBE. They also developed a machine learning...cellular processes that would cause DSBs, and its inclusion significantly reduced indels generated by CGBEs...complementary region of the non-target strand is excluded as ssDNA. DSB Double-Stranded Break A cut to...

..., and include information on tested antibodies against the target. Helpfully, this also includes clone...antibody for flow cytometry experiments. This will include a discussion of the Human Leucocyte Differentiation...approach Genetic knockout-based validation can conclusively prove the ability of an antibody to detect the...separation and reduce any background staining. This can include using: different blocking reagents; titration of...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown is...molecules expressed on human blood leukocytes, including antibodies against these markers. The 371 CD markers...

...sites are the most inclusive markers of regulatory regions in the genome, including enhancers, promoters...anywhere in the genome. Aneesh's research interests include proteomics, machine learning, and visualization...maps to find all coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might...project, find enhancers in embryonic stem cells, and conclude with a targeted CRISPR screen that can disrupt...screens for enhancers, check out the Appendix. Conclusion: Shedding light on dark matter Precise knowledge...

...chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a chimeric 5'...plasmids created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet...lentiviral packaging vector, a transfer vector including the gene of interest, and the plasmid coding for...is used; several common laboratory cell lines including 293 were immortalized with adenoviral protein ... stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral vectors...

... activation (CRISPRa) tools for neuroscience, including transgenic mice, neuron-optimized viral vectors... large size of Cas9 activators, which largely precludes delivery by adeno-associated vectors (AAV). This... express Cre under neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII;... in primary rat neurons over other promoters, including ubiquitous ones such as EF-1α and CAG. ... the differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes, provided...applications for which CRISPRa is well-suited, including studying mRNA dosing effects, cellular reprogramming...

...chemically distinct subcellular environments, including the organelles that compose the secretory pathway... significant post-translational modifications including glycosylation, disulfide bond formation, and proteolytic...real consequences of misfolded dark FP fusions including erroneous quantitation of fusion protein levels...imaging in a variety of cellular compartments including the secretory pathway (Figure 2), the inner membrane... Anatomy and Structural Biology. His interests include the quality control of secretory proteins in the... PMC2172584. 11. Jokitalo, Eija, et al. "Golgi clusters and vesicles mediate mitotic inheritance independently...