...how both the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...physically hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher...affect their function (the gut twists and contorts during the digestion of food, while brain tissue is relatively...System for Wireless, Programmable Neuromodulation During Free Behavior. 2020 42nd Annual International Conference...

.... We find it useful to start with a screening procedure in easily genetically modified cell types (top..., D., McDowell, I., Southern, K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards... C. (2023). Scaling of an antibody validation procedure enables quantification of antibody performance...., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A. M., Healy, L. M., Robertson...Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene...

...based green card applications and approved 91,023 during the first 3 quarters of 2017. As an international...have different roles, titles, and responsibilities during their careers - being aware of this can help take...Networking is extremely important for candidates during a job search and it is even more critical for international...for Neuroscientists to network with other members during the poster sessions, panels, exhibit floor, etc...and connect with people who could be beneficial during a career search. Doing these simple things will...

...Discussing data with colleagues can be more difficult during the pandemic era, with fewer opportunities for ...   Implement new tools for creative interaction During Zoom meetings, different tools can be used to promote...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes...some degrees of belonging that was probably lost during these weird times of shift working and social distancing...

...other to be physically present at certain times during the day? We asked three lab heads how they go about...will provide a solid foundation on which to thrive during time spent as a graduate student or postdoc. Although...chosen research field is important, the end goal during this time is to be trained to think and execute...to ask lab heads, lab members and lab neighbors during the interview process. What if I want a non-academic...

..., too. And remember, if you discover during grad school or during your postdoc that you enjoy and have...know what kind of plate they were talking about during the meetings. And “plate” certainly wasn’t the ...experimental protocols. Clarify: Asking for clarification during a meeting can help get everyone on the same page...

...communication is briefly inhibited in the entire worm during regeneration, the worm creates two heads. Subsequent...channel. Upon activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A...low-intensity blue light pulse and stays open for a longer duration upon activation. p-mcherry-C1-iC++_CA - A slow...junction protein-mediated cues control polarity during planarian regeneration." Developmental biology ...

... a look at how the stabilized promoters perform during these types of fluctuations. When cells are dividing... can also cause changes in plasmid copy number. During the shift from exponential to stationary phase,...promoters were able to better modulate gene expression during shifts in growth rate as compared to a constitutive...To simulate the increase in plasmid copy number during the transition to stationary phase, Segall-Shapiro...

...protection! When centrifuging viruses (a necessary step during production), there is a significant risk for aerosol... via needlestick or cut. Following all sharps procedures for your organization will significantly reduce...best to be well-rested and alert the day of the procedure. Mice move much more quickly than cell cultures...understandably induce some nerves. Following safety procedures and practicing safety culture, however, can let...

...special considerations. Conventional flow cytometry During conventional flow cytometry, light emitted by a...distinguish these two fluorophores from one another during spectral unmixing.     Figure 2: The ...telling exactly how many Cy7 molecules are added during that particular batch. This can present particular...upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical to include...

...temperature. Taq was first identified in the late 1960s during research at hot springs in Yellowstone National...programmed thermocycler. For more details on this procedure check out our PCR protocol page, protocol video... common for dNTPs and other reagents to run low during the final cycles which would slow or stop PCR amplification...this fluorescent dye, the concentration of the DNA during the PCR reaction cycles is continuously detected...

... It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies...temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

...plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation process, ...never receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria...while amp only affects the cell wall synthesis step during cell division. This is nifty because the recovery...it will induce mutations in the host/plasmid DNA during selection if it’s not completely bound up. While...

...defined chunks, called stories, and work on stories during short periods of time called sprints. The team ...different technical approaches that may come up during discussion.  One example of agility at work is ...we recently reduced the number of steps involved during checkout. Demo details for this would be something...developing new features and fixing bugs: we take time during each sprint to do some maintenance work on our ...

...magnetic beads and then incubated with a protein mix. During the incubation the immobilized antibody binds to...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...proteins from the sample prior to beginning the IP.  During the IP, the target protein in the sample binds ...weakly bound proteins in the sample are removed during a series of wash steps following the incubation...

...who referred to her lab as her family three times during a 10 minute conversation. Leaving a tight-knit ... “I’m a scientist.”    What should you expect during the lab → office transition? I’ve already provided...science,” and virtually every person you talked to during the day was also a scientist. But now you also ...upsetting, there are ways to get back to the bench during your off hours. DIY bio labs are cropping up in...

...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...for the entire translation process. For example, during high growth rate conditions, E. coli preferentially...biosynthetic enzymes may be preferentially charged during amino acid starvation thus resulting in higher ...

...time I’ve had a role in organizing collaborations. During my graduate studies at Karolinska Institutet I ...what was expected, and keeping track of speakers. During your graduate or postdoctoral work there are loads.... I am glad that I was able to build experience during my graduate studies in regard to reaching out to...Transferable Skills Guide Developing Transferable Skills During Science Training Resources on Addgene.org Check...

...structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered and evolved optimizations...2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM...can be damaged at the air-water interface (AWI) during the freezing process, resulting in sample loss....-water interface protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238...

...cells with physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles...can be critical when, say, the gene is essential during development and a knockout is not feasible or when...strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development (Aljiboury... and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development. PLoS...