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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 77 results for: EXO

Showing: 21 - 40 of 77 results

  1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule imaging TagBiFC technique...provides maximum flexibility in color labeling with exogenous fluorophores, which can even be bright enough ...

  2. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...inserted a linker and GFP tag at the end of the last exon of the gene to ensure it would be transcribed and...inserting the tag and the linker preceding the first exon of the gene. In this manner, we have created an ...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...), 3’->5’ exonuclease activity (increases amplification fidelity), and no 5’->3’ exonuclease activity ...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...we’ll add loxP sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model...targeting sites at least 100 bp away from the target exon on either side. They have successfully used guides...

  6. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Hopkins University, where he is trying turn a few exoplanets into a successful PhD thesis. We use the same...same atmospheric physics to understand exoplanets that we use to understand the Earth! Denver, CO, USA 39.7392...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding motif protein X-linked ...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...inhibiting this complex may allow time for 5′ flap exonucleases and DNA ligases to act first. PE4 and PE5, as...editing in mammalian cells: the small RNA-binding exonuclease protection factor La. They found that La, which...

  16. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Membrane receptors and ion channels are often exogenously expressed and studied in HEKs due to the cell...

  17. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...Professor who was always “off flying his plane to exotic places while occasionally emailing us his worldly...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for example. Some customization...
Showing: 21 - 40 of 77 results