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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 119 results for: Endog

Showing: 21 - 40 of 119 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally, dimeric Dronpa is... a target for MEK1 phosphorylation, much like endogenous ERK, and its localization serves as an indicator.... Zhou et al studied the negative feedback on endogenous MEK1/2 phosphorylation by creating a short pulse...

  2. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...conjugation. 1) Endogenous lysine residues, which can be found throughout the antibody. 2) Endogenous cysteines...the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...

  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...cell which will recombine the template with the endogenous gene. Here, we'll describe the techniques and...Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression GFP to track...responds. You can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously in many cell types, while shRNA and siRNA are... viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The...structures to create a mature siRNA duplex. Fun fact! Endogenous miRNA production starts with transcription of...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... rely on exogenous enzymes and those that use endogenous homologous recombination. Modifying bacterial...and homologous recombination As an alternative, endogenous homologous recombination enzymes can also accomplish... To allow manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS ...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can be ...Although similar, amiRNAs are engineered from endogenous precursors of microRNAs found in the genome, ...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...encoded, photocaged Cas9 system could silence endogenous gene expression - demonstrating light-induced...2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods 16:633–639...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors are prone... and promoter demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures...Virus-Based Vector Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed...

  9. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system can now ...Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states." Nature....

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...different GPCRs. This collection can be used to study endogenous ligands, the pathways they activate, as well ...

  11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...GFP. If there’s GFP expression, presumably the endogenous target site will be cut and repaired by NHEJ ...exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted cells (...

  13. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...sample where your secondary antibody recognizes endogenous antigens. In these cases, even though one full...purposes, since the fragment could bind not only the endogenous proteins you are trying to block but your primary...

  14. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ... the bait. Also, RaPID can’t be used to study endogenous RNAs at their physiological expression levels...levels; however, knock-in of BoxB sites into endogenous DNA sequences could allow one to study RNAs expressed...

  15. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...signaling can also modify other properties such as endogenous gene expression, which can lead to changes in...with the goal of creating receptors with less endogenous activation and reduced off target activation ...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...pegRNAs (but not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous GPCR. Example ...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...methods are not necessarily suitable for combining endogenous sequences. Table 2: High Throughput Cloning Strategies...modules themselves (so may not be suitable for endogenous coding sequences), so enzyme choice is critical...

  19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel. Unlike ...required as the small-molecule binding motif targets endogenous protein (Figure 1f). Labeling strategy pros...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...typically expressed at much higher levels than endogenous proteins. Data obtained from experiments using...tagged protein may not be translatable to the endogenous system and will need to be verified using alternative...
Showing: 21 - 40 of 119 results