... Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally, dimeric Dronpa is... a target for MEK1 phosphorylation, much like endogenous ERK, and its localization serves as an indicator.... Zhou et al studied the negative feedback on endogenous MEK1/2 phosphorylation by creating a short pulse...

...conjugation. 1) Endogenous lysine residues, which can be found throughout the antibody. 2) Endogenous cysteines...the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...

...cell which will recombine the template with the endogenous gene. Here, we'll describe the techniques and...Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression GFP to track...responds. You can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid...

... rely on exogenous enzymes and those that use endogenous homologous recombination. Modifying bacterial...and homologous recombination As an alternative, endogenous homologous recombination enzymes can also accomplish... To allow manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS ...

...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can be ...Although similar, amiRNAs are engineered from endogenous precursors of microRNAs found in the genome, ...

...encoded, photocaged Cas9 system could silence endogenous gene expression - demonstrating light-induced...2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods 16:633–639...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...

...transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors are prone... and promoter demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures...Virus-Based Vector Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed...

...it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system can now ...Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states." Nature....

...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...different GPCRs. This collection can be used to study endogenous ligands, the pathways they activate, as well ...

...GFP. If there’s GFP expression, presumably the endogenous target site will be cut and repaired by NHEJ ...exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted cells (...

...sample where your secondary antibody recognizes endogenous antigens. In these cases, even though one full...purposes, since the fragment could bind not only the endogenous proteins you are trying to block but your primary...

... the bait. Also, RaPID can’t be used to study endogenous RNAs at their physiological expression levels...levels; however, knock-in of BoxB sites into endogenous DNA sequences could allow one to study RNAs expressed...

...our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary... hr of treatment. In some primary cell lines, endogenous TRIM21 is sufficient for TRIM-Away, and you only...

...pegRNAs (but not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

...signaling can also modify other properties such as endogenous gene expression, which can lead to changes in...with the goal of creating receptors with less endogenous activation and reduced off target activation ...

...heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous GPCR. Example ...

...methods are not necessarily suitable for combining endogenous sequences. Table 2: High Throughput Cloning Strategies...modules themselves (so may not be suitable for endogenous coding sequences), so enzyme choice is critical...

...conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel. Unlike ...required as the small-molecule binding motif targets endogenous protein (Figure 1f). Labeling strategy pros...

...abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various...efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient enough to hinder adenine...

...the target antigen. Some examples could be an endogenous sample of wild type cells, purified protein, ...be sure to include a sample that expresses the endogenous protein, since folding of the recombinant protein...