We narrowed to 40 results for: Eomes

1. Sleeping Beauty Awakens for Genome Engineering

   Type

   Blog Post

   Published

   June 30, 2015, 2 p.m.

   ...with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein...

2. Plasmids 101: Antibiotic Resistance Genes

   Type

   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ... cell wall synthesis Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ... MCS for cloning X gene, an SV40 promoter, and Neomycin selection.   Table 1: Gamma-retrovirus glossary...

4. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...Proximity-dependent labeling has become a powerful approach for proteomic mapping in both cell culture and whole organisms...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   .... It also gives cells antibiotic resistance to neomycin so that you can select the population of cells...

6. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ...visualization of the whole de novo synthesized proteome. However, FUNCAT alone cannot be used to determine...

7. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   .... Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells was the...

8. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...CRISPR101: Editing the Epigenome  Mapping the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read...

9. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ... the Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more...

10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce cell death...

11. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...binding partners at numerous genomic loci through proteomics and the chromosome conformation capture (3C) ...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...Merabet S (2018) Generation of a versatile BiFC ORFeome library for analyzing protein–protein interactions...

13. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset based on...

14. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them. ...

15. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ...

16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    .../10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). ...

17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635...

18. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...fourth-generation DNA sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID...

19. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting...

20. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...Furthermore, tumor cells alter their cell-surface proteome to inhibit expression of co-stimulatory molecules...