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We narrowed to 237 results for: FAS

Showing: 21 - 40 of 237 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579...produced the three separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively...Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red) ...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors... https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity...Lab and collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage...can be used in different illumination regimes with fast activation kinetics and high responsivity near the...Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage indicator with enhanced sensitivity...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...transfer at the subcellular level requires a probe with fast kinetics and high signal/noise ratio. Here, Takeharu...Figure 7: B-gTEMP temperature-sensitive probe enables fast measurement of temperature and local heat transfer...

  4. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...captured in a single camera exposure. This is much faster than relying on raster scanning as is done in confocal...characteristic to capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...sample is then collected and projected back to a fast, sensitive detector such as a scientific CMOS camera...planes of a sample may also be useful for providing fast imaging along with optical sectioning (5). One such...preferred technique for many of these samples due to its fast temporal resolution and decreased phototoxicity....

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same level of control...design.   Optogenetics Chemogenetics Timing Fast activation/deactivation Prolonged activation Targeting...

  6. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. 4. Stem...after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene scientist...engineering technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the...you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University... Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University of...Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and ...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...Highly similar sequences (megablast)”, which is very fast and works best when the identity between your sequence... [Our QC process would be trouble-free and much faster if 95% of our results were always correct!]  Since...base pair from your results. This option is not as fast as megablast, but can return longer alignments to...

  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... The pEcCas plasmid can be operated at 37 ℃ for faster growth of its E. coli host, and the addition of...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids...the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal integration...on developing microbial genome editing tools for faster construction or reprogramming of cells to understand...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline of individual steps ...could iterate our development and testing cycle faster due to the ease of jumping back and forth between... For example, our bbduk process created trimmed FASTQ files as output, which we specified as input to ...could adjust the compute resources when needed, for faster runtime and decreased cost compared to purchasing...

  11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see Cre-lox ...Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how genes and proteins coordinate...9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls ...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to... tasks, and then executing on them in a timely fashion while allowing for creative solutions to emerge...some of the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...case, consulting can sometimes be challenging. The fast paced nature of projects often means working long...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...coli multiplexing plasmid is available from the Koffas lab. Scaling up your multiplexed gRNAs with Golden... on a separate plasmid. Multiplexing in E. coli Koffas Lab CRISPathBrick Multiplex Plasmid: This system... E. coli CRMAGE Plasmids: The CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based...possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed...

  14. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...with a confocal microscope, yet I was once again fascinated by the world that reveals itself when looking...by the ASM Agar Art Winners! It is absolutely fascinating to see the living art designed by creative minds...steady hand. By connecting art and science, a fascinating interface is created that produces loads of inspiration...

  15. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used by Addgene is ...approvals, so your plasmid requests can get to your lab faster. For Everybody: Friendly Support: Have a question...

  16. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...example, the far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal ...surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a tdTomato fluorescent...in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... Alter chromatin architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing...

  17. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...is activated eight times faster than GRABNE and deactivated three times faster. This enhanced response ...sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked...

  18. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...to the grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved...coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited materials...

  19. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...Addgene does a lot to ensure that we can facilitate a fast and agile MTA process. This is important to keep... last twenty years! This has helped us develop a fast, agile approach, which we’ve updated and refined...organization upon purchase. We recognize exceptionally fast tech transfer offices with the Golden Stopwatch ...

  20. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...primary antibodies). Direct westerns are simpler and faster than indirect ones and can be used in highly quantitative...for very large proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is...various manufacturers. These proprietary systems are fast (often <10 minutes) and simple, utilizing individually...fluorescent proteins. Chemiluminescence enzymes are cheap, fast, shelf-stable before activation, and light-insensitive...
Showing: 21 - 40 of 237 results