We narrowed to 619 results for: LIS;

... reliable cloning technique that was developed by Li, et al., in 2011. This method is ligation independent...Biology 38 (2016): 145-154. PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified...

... https://doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the...check out this review article published in Reviews in Medical Virology (Li et a., 2018).   Figure...

...recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods. 2007 Mar;4(3):251-6....method for sequence- and ligation-independent cloning. Li MZ, Elledge SJ. Methods Mol Biol. 2012;852:51-9. ...

...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...of an accessible supply chain for parts that are listed in the bill of material (BOM) of an assembly, the...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

... consider FastCloning, first described by Chaokun Li and colleagues. Here you simply PCR-amplify your ...finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified...number is a bit smaller, let’s say 4 or less. The list goes on... In this post, we’ve reviewed many of ...

....2014.09.039 Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying...dCas9, also referred to as CRISPRa, was initially published in 2013 (Bikard et al., 2013, Perez-Pinera et ...

...result from excessive nuclease activity in tissues (Li et al., 2018). 4. Selection marker while engineering....e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...that blocks type II-A CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-...

...critical that the unknown sample’s concentration lies within the dynamic range of the curve. The dynamic...trendline, which may be good enough if your unknown lies within the linear portion of the graph. It does ...resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control for All ...viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in food; and...BCA assays have linear trendlines while those for ELISAs and other enzymatic assays are sigmoidal. Once ...

...2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin substantially... 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, ...efforts. This holds true for any prime editor experimentalist, and especially shines once large-scale prime...

...different leaf infiltration methods Co-cultivation (Li et al., 2009): The co-cultivation of Agrobacterium...Humana Press, 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified...precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily...might help to get you started. Once you have established a well working protocol in your lab, it can become...

... a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the Codon...683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of...

...s13039-018-0368-2  Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE,...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of...

...editing using TnpB. The main difference between them lies in the choice of promoter for ωRNA (reRNA) expression...., Liu, S., Zhang, X., Hu, W., Wang, Y., Han, C., Li, D., & Wang, X. (2025). Transgene- and tissue culture-free...sequence 5′-TTGAT-3′, while TnpB from Deinococcus geothermalis (Dge10TnpB) targets a different TAM (5′-TTAT...plasmid is now ready for Agrobacterium-mediated, biolistic, or PEG-mediated transformation into plant cells...

...Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG,... SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

...past and present, but the real promise of Addgene lies in our future. As we near our 20th anniversary, ...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...

...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method ...PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted...short list of some other common applications and links to relevant papers and webpages are listed below...such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the... EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay) Southern/Northern... Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation and single-step ...

...Lloyd, K., Jardine, J., Coppola, J., Meijers, R., Li, J., & Springer, T. A. (2024). Synthetic integrin...https://doi.org/10.1101/2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé, V., Hao, Y., Coppola, J., Yan, J...recommended for use in flow cytometry. Based on the established applications of the original hybridoma monoclonals...

...adjacent motif (PAM). ABE7.10 can edit target 'A' if it lies in a small genomic window: at protospacer position...Nature plants (2018): 1. PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and ...

...in bioluminescent organisms, but their real power lies in the clever ways scientists have adapted them ...

...Zhang X, Su J, Jeong M, Gundry MC, Huang Y-H, Zhou Y, Li W, Goodell MA (2017) Targeted DNA methylation in ...publication at Addgene. Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...are also available as controls. For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR...