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We narrowed to 109 results for: LYC

Showing: 21 - 40 of 109 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...targets human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library... PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...protein folding, formation of disulfide bridges, glycosylation and acetylation to create functional, stable...systems enable high expression of very complex, glycosylated proteins that cannot be produced in  E. coli...optimized. Yeast cells are also known for hyperglycosylating proteins which may be an issue depending ...

  5. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ... Addgene - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control...storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture... to transformed cells and finally to bacterial glycerol stocks that are used to fulfill orders.  The barcoded...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  7. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes uracil...uracil-labeled template strand is degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). ...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

  14. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly...higher plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give...

  15. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across both systems.  ...Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  19. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...
Showing: 21 - 40 of 109 results