Antibodies 101: Multiplex Immunofluorescence
Type
Blog Post
Published
May 23, 2023, 1:15 p.m.
...different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1...Secondary antibody
SCN1A sodium channels
anti-pan-Nav R-mAb K58/35R (Mouse IgG2a)
Goat anti-mouse...anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image...). Even better, show each channel in individual panels, then the multicolor overlay; this helps all viewers...