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We narrowed to 462 results for: PAN

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Showing: 21 - 40 of 462 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow was also used in zebrafish...Nature 450.7166 (2007): 56. PubMed PMID: 17972876. Pan, Y. Albert, et al. "Multicolor Brainbow imaging in...cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature pigmented melanocytes...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1...Secondary antibody SCN1A sodium channels anti-pan-Nav R-mAb K58/35R (Mouse IgG2a) Goat anti-mouse...anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image...). Even better, show each channel in individual panels, then the multicolor overlay; this helps all viewers...

  5. Deaminet 2026: Breakthroughs in Base Editing, Deaminase Biology, and Therapeutic Translation

    Type
    Blog Post
    Published
    March 10, 2026, 1:14 p.m.
    ...License. oDNA: Immune-evasive DNA donors for pan-mutation editing Benjamin Kleinstiver (Massachusetts...integration. The oDNA approach addresses a key barrier for pan-mutation editing strategies by enabling large sequence... prime editing toward the clinic. Across talks spanning mechanism, off-target biology, and in vivo delivery...in 2021, yet both collections have continued to expand steadily. One striking pattern is the longevity...changes enabled by prime editing. As the toolkit expands to include novel deaminase families (mSCD, DYW)...doi.org/10.64898/2025.12.21.695777 Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...for neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want to consider one...., et al.,Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361,1259-1262(2018). ... on the Addgene blog The PAM Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant...

  8. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ....2022.09.015 Ramani, B., Rose, I. V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., ...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair...

  12. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales, C., ...

  13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang...-cells were isolated from healthy individuals, expanded, and infected with HIV-1. For two HIV-1 strains...especially difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove...

  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...125.27 (2003): 8074–8075. PubMed PMID:12837056. 8. Pan, J. feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application...

  16. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020...part of the world manifesting as a full-fledged pandemic. Containing the spread of this virus has become...widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR tests to diagnose...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020...standard laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of ...molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started based on DETECTR...

  19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach... last, and more importantly, if it does extend lifespan. This work should be done in mouse and large animal...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat... offers additional PAM options that can greatly expand gRNA options. Created with BioRender.com...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best...10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM...
Showing: 21 - 40 of 462 results