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We narrowed to 181 results for: PID

Showing: 21 - 40 of 181 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... is relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...Studies on the kinetics of these sensors showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, ... genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...the viral genome and proteins. This is, for the intrepid researcher looking for molecular biology tools...protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid, called an envelope...copies, which are released from the cell like baby spiders from an egg sac. (Okay, some enveloped viruses ...

  3. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine...oxysterols, bile acids, and numerous xenobiotic lipids derived from the diet. Additional members of this...Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866...

  4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome replication more...types of fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before...T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL...

  5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...levels? Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away...antibody targeting the protein of interest led to rapid, targeted degradation that is dependent on proteasome...FIRE–Cas9), combines these approaches to enable rapid and reversible recruitment of chromatin complexes...

  6. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...receptors and transporters, are integrated into lipid bilayers. Fusion of an FP to a transmembrane protein...localized to the endoplasmic reticulum, the normally spiderweb-like pattern of tubules can be grossly distorted...PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...W. (1983). Worldwide epidemiology of human adenovirus infections. Am J Epidemiol, 117(4), 455–466. https...was first isolated from human adenoid tissue and rapidly caused the death of adenoid cells in vitro (Rowe...

  8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...is because organic solvents can be quite harsh - lipids and soluble proteins can be lost during fixation... others. That said, organic solvents’ impact on lipids means that they are able to permeabilize your sample...examples of non-ionic detergents that interact with lipids and proteins in membranes and permeabilize non-...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...cell line. This design allows highly localized, rapid and reversible microtubule severing and has very... sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidylcholine (PC), the predominant membrane lipid, to phosphatidic acid (PA) or it can swap phosphatidylchonine...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...research project was hard; I was researching the epidemiology of breast cancer and I loved the practical aspects...would advise you to be wary of companies which are rapidly expanding, as this might not be sustainable. After...

  11. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...software could also be used as a simple tool to rapidly find one or a few gRNAs suggested to be effective...appropriate phenotypic readout, similar mapping could be rapidly performed by targeting all potential components...identified for each gene. This file can be used to rapidly get an overview of the list of gRNAs generated....

  12. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...probe into a single molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces...chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr ...brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely...

  13. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...advancing this field and how we are able to support rapid progress in all areas of research. We welcome your...

  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...of each construct. This new tool kit allows the rapid generation of stable transgenic cell lines which..., et al. Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Biotechnol...

  16. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...figure below). Subsequent exposure to red light rapidly deactivates the system. Voigt’s lab used this system...GFP reporter. Subsequent exposure to red light rapidly deactivates the system. Version 1 of this far-red...

  17. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...to tackle a controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR...Harley and Lexi, and a clutter ofoutdoors-only spiders, all of whom are named Charlotte, all of whom are...

  19. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...have a thinner peptidoglycan membrane and an outer lipid membrane. Common gram negative bacteria include ...negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan membrane...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...
Showing: 21 - 40 of 181 results